Spatial and temporal expression profiles suggest the involvement of gelatinase A and membrane type 1 matrix metalloproteinase in amphibian metamorphosis
Spatial and temporal expression profiles suggest the involvement of gelatinase A and membrane type 1 matrix metalloproteinase in amphibian metamorphosis
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DOI：
10.1007/s00441-005-0099-7
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发表时间：
2006-04-01
影响因子：
3.6
通讯作者：
Shi, YB
中科院分区：
文献类型：
--
作者：
Hasebe, T;Hartman, R;Shi, YB
基质金属蛋白酶（MMP）是能够降解细胞外基质（ECM）的各种组分的蛋白酶家族。其中，膜型MMP-1（MT 1-MMP）参与了MMP明胶酶A（GelA）的活化，提示它们可能在发育和发病中共同发挥作用。在这里，我们已经调查了时空表达谱非洲爪蟾MT 1-MMP和GelA基因在甲状腺激素依赖性变态。我们的研究集中在两个器官上：（1）肠，它首先经历蝌蚪上皮细胞通过凋亡的退化，然后是成体上皮细胞和其他组织的发育，和（2）尾巴，它通过程序性细胞死亡完全吸收。我们发现，MT 1-MMP和GelA都上调肠和尾巴时，这两个器官进行变态。在器官内，MT 1-MMP和GelA在自然和甲状腺激素诱导的变态过程中在结缔组织中共表达。此外，MT 1-MMP（而不是GelA）也表达在变形肠的纵肌细胞。这些结果表明，MT 1-MMP和GelA功能一起在ECM降解或重塑与变态和MT 1-MMP有额外的GelA独立的作用，在成人肠纵肌的发展。
The matrix metalloproteinases (MMPs) are a family of proteases capable of degrading various components of the extracellular matrix (ECM). Among them, the membrane type MMP-1 (MT1-MMP) has been shown to participate in the activation of MMP gelatinase A (GelA), suggesting that they may function together in development and pathogenesis. Here, we have investigated the spatiotemporal expression profiles of Xenopus laevis MT1-MMP and GelA genes during thyroid-hormone-dependent metamorphosis. We have focused our studies on two organs: (1) the intestine, which undergoes first the degeneration of the tadpole epithelium through apoptosis and then the development of adult epithelium and other tissues, and (2) the tail, which completely resorbs through programmed cell death. We show that both MT1-MMP and GelA are upregulated in the intestine and tail when both organs undergo metamorphosis. Within the organs, MT1-MMP and GelA are coexpressed in the connective tissues during both natural and thyroid-hormone-induced metamorphosis. In addition, MT1-MMP (but not GelA) is also expressed in the longitudinal muscle cells of the metamorphosing intestine. These results suggest that MT1-MMP and GelA function together in the ECM degradation or remodeling associated with metamorphosis and that MT1-MMP has additional GelA-independent roles in the development of adult longitudinal muscle in the intestine.