Isolation studies reveal a shift in the cultivable microbiome of oak affected with Acute Oak Decline
Isolation studies reveal a shift in the cultivable microbiome of oak affected with Acute Oak Decline
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DOI：
10.1016/j.syapm.2016.07.002
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发表时间：
2016-10-01
影响因子：
3.4
通讯作者：
McDonald, James E.
中科院分区：
文献类型：
--
作者：
Denman, Sandra;Plummer, Sarah;McDonald, James E.
急性橡树衰退是英国橡树衰退综合征中的一种综合征。大量的茎出血和幼虫画廊的本地蟾蜍，Agrilus biguttatus的特点是疾病。进行了一项比较健康与患病树木的系统研究。这项工作报告的结果隔离健康的树木，患病和非症状组织内的AOD影响的树木，在英格兰的五个网站。使用DNA促旋酶B基因或ITS 1测序鉴定细菌和真菌。患病组织（82%）的比例显着高于健康组织（18%）或患病树中的无症状组织（33%）。整体细菌群落组成在每个网站不同，但显着的相似性是显而易见的，在所有网站的患病组织。病树以肠杆菌科为主，健康树以假单胞菌科为主。患病组织与某些细菌物种之间存在显著关联，这意味着组织坏死的原因不是由于随机微生物群。Brenneria goodwinii和Gibbsiella quercinecans是从患病组织中一致分离的关键种; Rahnella victoriana和未命名的假单胞菌分类群也经常从健康和患病树木中分离出来。大多数真菌分离的外树皮，并没有显着的树木健康状况。得出的结论是，在患病树木的可培养细菌微生物组中存在转变，肠杆菌科在有症状的组织中强烈存在，但在健康组织中不存在。没有一个单一的物种占主导地位的分离从患病组织和组织降解，因此，在AOD可能有一个多微生物的原因。Crown版权所有（C）2016由Elsevier GmbH发布。All rights reserved.
Acute Oak Decline is a syndrome within the Oak Decline complex in Britain. Profuse stem bleeding and larval galleries of the native buprestid, Agrilus biguttatus characterize the disease. A systematic study comparing healthy with diseased trees was undertaken. This work reports the result of isolations from healthy trees, diseased and non-symptomatic tissue within AOD affected trees, at five sites in England. Bacteria and fungi were identified using the DNA gyrase B gene, or ITS 1 sequencing. A significantly higher proportion of diseased tissues (82%) yielded more bacteria than either healthy (18%) or non-symptomatic tissue in diseased trees (33%). Overall bacterial community compositions varied at each site, but significant similarities were evident in diseased tissues at all sites. Enterobacteriaceae dominated in diseased trees whereas Pseudomonadaceae dominated healthy trees. Significant associations between diseased tissues and certain bacterial species occurred, implying that the cause of tissue necrosis was not due to random microbiota. Brenneria goodwinii and Gibbsiella quercinecans were key-species consistently isolated from diseased tissue; Rahnella victoriana and an un-named Pseudomonas taxon were also frequently isolated from both healthy and diseased trees. Most fungi isolated were from the outer bark and had no significant association with tree health status. It was concluded that there was a shift in the cultivatable bacterial microbiome of diseased trees, with Enterobacteriaceae strongly represented in symptomatic but not healthy tissues. No single species dominated the isolations from diseased tissues and the tissue degradation in AOD is therefore likely to have a polymicrobial cause. Crown Copyright (C) 2016 Published by Elsevier GmbH. All rights reserved.