UME6 is a key regulator of nitrogen repression and meiotic development.
UME6 is a key regulator of nitrogen repression and meiotic development.
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DOI：
10.1101/gad.8.7.796
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发表时间：
1994-04
影响因子：
10.5
通讯作者：
Randy Strich;Richard T. Surosky;Camille M. Steber;Evelyne Dubois;F. Messenguy;Rochelle Easton Esposito
中科院分区：
文献类型：
--
作者：
Randy Strich;Richard T. Surosky;Camille M. Steber;Evelyne Dubois;F. Messenguy;Rochelle Easton Esposito
该报告描述了UME6（CAR80/CARGRI）的鉴定，克隆和分子分析，这是早期减数分裂基因表达的关键转录调节剂。 UME6功能的丧失导致在营养生长期间的早期减数分裂转录本的完全压缩水平（高于基础水平高70至100倍）。相反，先前鉴定出的UME基因座（UME1至UME5）的突变导致早期减数分裂基因的低至中度压缩（增加2至10倍）。插入和缺失等位基因的行为表明UME6对于有丝分裂分裂是可分配的，但需要减数分裂和孢子发芽。尽管在营养生长过程中，减数分裂基因的表达水平很高，但仅略微降低了UME6突变体单倍体和二倍体细胞的产生时间。但是，ASCUS的形成和孢子生存能力都受到更严重的影响。 UME6基因编码一个91 kD蛋白，该蛋白包含类似于GAL4的DNA结合域的C6锌簇基序。 UME6函数需要该域的完整性。据报道，CAR80突变未能补充UME6的插入等位基因。 CAR80是精氨酸分解代谢酶的氮抑制所需的基因。在这里，通过序列分析，我们证明了UME6和CAR80是相同的。在氮饥饿和减数分裂发育过程中对UME6 mRNA的分析表明，其转录是组成型，表明对UME6活性的调节发生在转录后水平。
This report describes the identification, cloning, and molecular analysis of UME6 (CAR80/CARGRI), a key transcriptional regulator of early meiotic gene expression. Loss of UME6 function results in the accumulation of fully derepressed levels (70- to 100-fold increase above basal level) of early meiotic transcripts during vegetative growth. In contrast, mutations in five previously identified UME loci (UME1 to UME5), result in low to moderate derepression (2- to 10-fold increase) of early meiotic genes. The behavior of insertion and deletion alleles indicates that UME6 is dispensable for mitotic division but is required for meiosis and spore germination. Despite the high level of meiotic gene expression during vegetative growth, the generation times of ume6 mutant haploid and diploid cells are only slightly reduced. However, both ascus formation and spore viability are affected more severely. The UME6 gene encodes a 91-kD protein that contains a C6 zinc cluster motif similar to the DNA-binding domain of GAL4. The integrity of this domain is required for UME6 function. It has been reported recently that a mutation in CAR80 fails to complement an insertion allele of UME6. CAR80 is a gene required for nitrogen repression of the arginine catabolic enzymes. Here, through sequence analysis, we demonstrate that UME6 and CAR80 are identical. Analyses of UME6 mRNA during both nitrogen starvation and meiotic development indicate that its transcription is constitutive, suggesting that regulation of UME6 activity occurs at a post-transcriptional level.