Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes
Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes
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DOI：
10.1007/s00441-009-0848-0
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发表时间：
2009-10-01
影响因子：
3.6
通讯作者：
Denda, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Tsutsumi, Moe;Inoue, Kaori;Denda, Mitsuhiro
表皮中的钙动力学在屏障稳态和角质形成细胞分化中起着至关重要的作用。我们最近提出，角质形成细胞的电生理反应代表了皮肤感觉系统对环境刺激的前沿。在本研究中，我们已经评估了增殖和分化的人角质形成细胞对机械应力的反应，通过测量细胞内钙水平。在分化之前，机械应力在有限的区域内诱导钙波;这被腺苷三磷酸双磷酸酶完全阻断，腺苷三磷酸双磷酸酶降解ATP。在分化的角质形成细胞的情况下，钙波在更大的区域上传播。腺苷三磷酸双磷酸酶的应用不能完全抑制这种波。因此，在分化的细胞中，钙波的诱导可能不仅涉及ATP，而且还涉及另一种因素。免疫组织化学研究表明，缝隙连接蛋白26和43，这两个组成部分，表达在分化的角质形成细胞的细胞膜。应用辛醇或甘珀酸，阻断缝隙连接，显着减少分化的角质形成细胞中的钙波传播。因此，通过缝隙连接的信号可能参与诱导钙波响应于机械应力在表皮的上层。
Calcium dynamics in the epidermis play a crucial role in barrier homeostasis and keratinocyte differentiation. We have recently suggested that the electro-physiological responses of the keratinocyte represent the frontier of the skin sensory system for environmental stimuli. In the present study, we have evaluated the responses of proliferating and differentiated human keratinocytes to mechanical stress by measuring the intracellular calcium level. Before differentiation, mechanical stress induces a calcium wave over a limited area; this is completely blocked by apyrase, which degrades ATP. In the case of differentiated keratinocytes, the calcium wave propagates over a larger area. Application of apyrase does not completely inhibit this wave. Thus, in differentiated cells, the induction of calcium waves might involve not only ATP, but also another factor. Immunohistochemical studies indicate that connexins 26 and 43, both components of gap junctions, are expressed in the cell membrane of differentiated keratinocytes. Application of octanol or carbenxolone, which block gap junctions, significantly reduces calcium wave propagation in differentiated keratinocytes. Thus, signaling via gap junctions might be involved in the induction of calcium waves in response to mechanical stress at the upper layer of the epidermis.