DNA fingerprinting using PCR: a practical forensic science activity
DNA fingerprinting using PCR: a practical forensic science activity
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使用 PCR 进行 DNA 指纹识别：一项实用的法医学活动
DOI：
10.1080/00219266.2008.9656148
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发表时间：
2008
影响因子：
1.1
通讯作者：
J. Ahn
中科院分区：
文献类型：
--
作者：
Hyun;J. Ahn
本文介绍了一种适用于高等院校的法医学仿真程序。学生们被要求使用本研究中开发的简单协议通过DNA指纹识别找到假定的嫌疑人。从一根发根和一滴血中提取了DNA样本，这是法医科学中常见的DNA来源。DNA指纹图谱采用PCR扩增可变数串联重复（VNTR）序列，共5组引物。尽管学生之间的DNA指纹结果质量有所不同，但学生们通过正确识别嫌疑人，总体上成功地执行了该程序。该课程以探究为基础的问题解决方法有助于理解生物技术及其应用的基本概念。
This paper describes a forensic science simulation programme applicable for use in colleges. Students were asked to find a putative suspect by DNA fingerprinting using a simple protocol developed in this study. DNA samples were obtained from a hair root and a drop of blood, common sources of DNA in forensic science. The DNA fingerprinting protocol uses PCR to amplify Variable Number Tandem Repeat (VNTR) sequences with five set of primers. Although there was some variation in the quality of DNA fingerprinting results among students, the students generally carried out the program successfully by correctly identifying the suspect. The inquiry-based problem solving approach of this programme facilitates an understanding of the basic concepts of biotechnology and its application.