Experimental replacement of the thoracic esophagus with a bioabsorbable collagen sponge scaffold supported by a silicone stent in dogs
Experimental replacement of the thoracic esophagus with a bioabsorbable collagen sponge scaffold supported by a silicone stent in dogs
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DOI：
10.1097/00002480-199907000-00011
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发表时间：
1999-07-01
期刊：
影响因子：
4.2
通讯作者：
Tamura, N
中科院分区：
文献类型：
--
作者：
Yamamoto, Y;Nakamura, T;Tamura, N
我们制造了一种由胶原海绵制成的食管假体，由内部硅胶管支撑。胶原蛋白来源于猪皮，并通过脱水处理进行交联。我们植入我们的假体在9只狗切除5厘米长的胸食管。术后静脉高营养4周。29天后，通过内窥镜取出内硅胶管，并立即重新开始经口喂养。9只犬均存活1个月以上，3只存活1年以上。内窥镜下，再生食管的腔表面覆盖着光滑的粘膜，但在再生食管的中部区域观察到狭窄。显微镜下，术后1个月重建部位无异物残留;胶原海绵被吸收并被宿主组织取代。在3个月内观察到完全上皮化。粘膜肌层、食管腺和骨骼肌的再生被观察到在软骨附近。我们的人工食管有望在临床环境中进行食管重建，甚至在胸腔中。
We manufactured an esophageal prosthesis made of a collagen sponge supported by an inner silicone tube. The collagen was derived from porcine skin and was crosslinked by dehydrothermal treatment. We implanted our prosthesis in nine dogs after resecting a 5 cm length of the thoracic esophagus. Intravenous hyperalimentation was performed for 4 weeks after the surgery. After 29 days, the inner silicone tube was removed endoscopically and oral feeding was restarted immediately. All nine dogs survived more than 1 month, and three of the nine dogs survived for more than 1 year. Endoscopically, the luminal surface of the regenerated esophagus was covered with a lustrous, smooth mucosa, but stenosis was observed in the midregion of the regenerated esophagus. Microscopically, no foreign material remained at the reconstructed site 1 month after surgery; the collagen sponge was absorbed and replaced by host tissue. Complete epithelization was observed within 3 months. Regeneration of the lamina muscularis mucosae, esophageal glands, and skeletal muscle were observed near the anastomoses. Our artificial esophagus holds promise for esophageal reconstruction in a clinical setting, even in the thorax.