Identification of urinary metabolites in rats adminis- tered the fluorine-containing pyre- throids metofluthrin, profluthrin, and transfluthrin
Identification of urinary metabolites in rats adminis- tered the fluorine-containing pyre- throids metofluthrin, profluthrin, and transfluthrin
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给予含氟拟除虫菊酯甲氧氟菊酯、丙氟菊酯和四氟苯菊酯的大鼠尿液代谢物的鉴定
DOI：
10.1080/02772248.2012.729838
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Toshiaki Yoshida
中科院分区：
文献类型：
--
作者：
T.Doi;K. Kajimura and S. Taguchi;Toshiaki Yoshida
对最近广泛用作驱蚊剂或防蛀驱避剂的含氟拟除虫菊酯四氟菊酯、氟氯菊酯和甲氧氟菊酯给药后大鼠尿液中的主要代谢物进行鉴定，以建立评价这些拟除虫菊酯在一般人群中吸收量的生物学指标。雄性Sprague-Dawley大鼠分别腹腔内单次给予300 mg kg− 1体重的每种拟除虫菊酯，并采集尿样，直至给药后24小时。通过气相色谱-质谱法鉴定的代谢产物如下：2，3，5，6-四氟苄醇、2，3，5，6-四氟苯甲酸和3-（2，2-二氯乙烯基）-2，2-二甲基环丙烷羧酸（DCCA）用于四氟菊酯; 4-甲基-2，3，5，6-四氟苄醇、4-甲基-2，3，5，6-四氟苯甲酸、4-羟甲基-2，3，5，6-四氟苄醇（HOCH 2-FB-Al）和2，2-二甲基-3-（1-丙烯基）-环丙烷羧酸（MCA）; 4-甲氧基甲基-2，3，5，6-四氟苄醇、HOCH 2-FB-Al和MCA用于甲氧氟菊酯。此外，检测到几种估计为代谢产物的化合物，如下所示：四氟菊酯的羟基化DCCA及其内酯;四氟菊酯的4-羟甲基-2，3，5，6-四氟苯甲酸（HOCH 2-FB-Ac）、羟基化MCA及其内酯;甲氧氟菊酯的4-甲氧基甲基-2，3，5，6-四氟苯甲酸、HOCH 2-FB-Ac、羟基化MCA及其内酯。给药的拟除虫菊酯发生代谢反应，如酯水解和氧化，大多数代谢产物以其结合物的形式排泄到尿液中。这些研究结果应作为评价一般人群暴露于拟除虫菊酯的健康影响的基本数据。
The major metabolites in urine of rats administered the fluorine-containing pyrethroids transfluthrin, profluthrin, and metofluthrin, which are used widely recently as mosquito repellents or mothproof repellents, were identified to establish biological indexes for evaluating the absorption amounts of these pyrethroids in the general population. A single dose of 300 mg kg−1body weight of each pyrethroid was separately administered intraperitoneally to male Sprague-Dawley rats and urine samples were collected until 24 hours after the administration. The metabolites identified by gas chromatography-mass spectrometry were as follows: 2,3,5,6-tetrafluorobenzyl alcohol, 2,3,5,6-tetrafluorobenzoic acid, and 3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid (DCCA) for transfluthrin; 4-methyl-2,3,5,6-tetrafluorobenzyl alcohol, 4-methyl-2,3,5,6-tetrafluorobenzoic acid, 4-hydroxymethyl-2,3,5,6-tetrafluorobenzyl alcohol (HOCH2–FB–Al), and 2,2-dimethyl-3-(1-propenyl)-cyclopropanecarboxylic acid (MCA) for profluthrin; 4-methoxymethyl-2,3,5,6-tetrafluorobenzyl alcohol, HOCH2–FB–Al and MCA for metofluthrin. In addition, several compounds estimated to be metabolites were detected as follows: hydroxylated DCCA and its lactone for transfluthrin; 4-hydroxymethyl-2,3,5,6-tetrafluorobenzoic acid (HOCH2–FB–Ac), hydroxylated MCA and its lactone for profluthrin; 4-methoxymethyl-2,3,5,6-tetrafluorobenzoic acid, HOCH2–FB–Ac, hydroxylated MCA, and its lactone for metofluthrin. The pyrethroids administered underwent metabolic reactions such as ester hydrolysis and oxidation, and most of the metabolites were excreted as their conjugates into urine. These findings should be useful as basic data for evaluating the health effects of exposure to pyrethroids for the general population.