Anxiety-like behavior of young rats after 2-week zinc deprivation
Anxiety-like behavior of young rats after 2-week zinc deprivation
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DOI：
10.1016/j.bbr.2006.11.023
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发表时间：
2007-02-12
影响因子：
2.7
通讯作者：
Oku, Naoto
中科院分区：
文献类型：
--
作者：
Takeda, Atsushi;Tamano, Haruna;Oku, Naoto
对幼龄大鼠给予1 ~ 2周缺锌饮食，观察其脑内神经元功能与神经心理行为的关系。血清锌浓度低于对照组的50%。然而，体内微透析测定的海马灌注液中锌浓度在2周锌剥夺后没有下降。Timm染色在突触前囊泡中检测到组织化学反应性锌，在大脑中也没有减弱。另一方面，剥夺锌2周后，血清皮质酮浓度（上午测定）明显升高，海马细胞内钙信号（fura-2 AM测定）也升高。在缺锌的海马中，细胞内游离钙浓度可能在细胞外液锌浓度降低之前发生改变。在开场实验中，大鼠在2周的缺锌后，穿越线的次数和梳理毛发的时间都有所减少。在加迷宫测试中，锌缺乏的大鼠在张开的手臂上花费的时间也减少了，这表明锌缺乏的焦虑样行为增加了。目前的研究表明，锌缺乏症中焦虑样行为的增加可能与海马细胞中基底游离钙浓度的增加有关，这可能是由于血清皮质酮浓度的增加。(c) 2006 Elsevier B.V.版权所有
The relationship between neuronal function in the brain and neuropsychological behavior were analyzed in young rats fed a zinc-deficient diet for 1-2 weeks. Serum zinc concentration was less than 50% of that of the control. However, zinc concentration in the hippocampal perfusate measured by the in vivo microdialysis was not decreased after 2-week zinc deprivation. Timm's stain, with which histochemically reactive zinc in the presynaptic vesicle is detected, was not also attenuated in the brain. On the other hand, serum corticosterone concentration, which was determined in the morning, was markedly increased after 2-week zinc deprivation and intracellular calcium signal, which was determined by fura-2 AM, was also increased in the hippocampus. In the hippocampus in zinc deficiency, intracellular free calcium concentration may be altered prior to the decrease in zinc concentration in the extracellular fluid. When rats were subjected to the open-field test, the frequency of line crossing and the time of grooming were decreased after 2-week zinc deprivation. In the plus-maze test, the time spent in the open arms was also decreased in zinc-deficient rats, Suggesting that anxiety-like behavior is increased in zinc deficiency. The present study indicates that the increase in anxiety-like behavior in zinc deficiency may be linked to the increased concentration of basal free calcium in hippocampal cells, probably due to the increase in serum corticosterone concentration. (c) 2006 Elsevier B.V. All rights reserved.