Oxidative stress and antioxidant status in patients undergoing prolonged exposure to hyperbaric oxygen
Oxidative stress and antioxidant status in patients undergoing prolonged exposure to hyperbaric oxygen
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DOI：
10.1016/j.clinbiochem.2003.12.001
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发表时间：
2004-04-01
影响因子：
2.8
通讯作者：
Canestrari, F
中科院分区：
文献类型：
--
作者：
Benedetti, S;Lamorgese, A;Canestrari, F
目的：为了评估患者的氧化应激条件进行长时间暴露于高压氧（HBO）和可能的修改的抗氧化防御系统在没有抗氧化supplementation.Design和方法：12例患者暴露于15 HBO治疗的病理条件相关的缺氧，被列入本研究。在第1次和第15次HBO session.Results：HBO重复暴露导致血浆活性氧代谢产物（ROM）和丙二醛（MDA）的显著积累。15次高压氧治疗后，还原型谷胱甘肽（GSH）、α-生育酚和视黄醇血浆水平没有相关差异;然而，与第一次高压氧暴露相比，观察到红细胞超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性显著降低;谷胱甘肽过氧化物酶（GPx）活性几乎保持不变。在没有抗氧化剂补充的情况下，长时间的HBO治疗会导致氧化应激，这似乎特别影响酶抗氧化防御系统的反应;由氧活性物质引起的酶的化学修饰和随后的蛋白质失活之间的可能关系正在研究中。(C)2004年加拿大临床化学家协会。All rights reserved.
Objectives: To evaluate the condition of oxidative stress in patients undergoing prolonged exposure to hyperbaric oxygen (HBO) and the possible modifications of the antioxidant defense systems in the absence of antioxidant supplementation.Design and methods: Twelve patients exposed to 15 HBO treatments for pathological conditions related to hypoxia, were included in the study. Oxidative stress indices as well as plasma and erythrocyte antioxidant levels were measured in blood samples collected both at the 1st and 15th HBO session.Results: The repeated exposures to HBO led to a significant accumulation of plasmatic reactive oxygen metabolites (ROM) and malondialdehyde (MDA). After 15 HBO sessions, no relevant differences were detected for reduced glutathione (GSH), alpha-tocopherol, and retinol plasma levels; however, a significant decrease in erythrocyte superoxide dismutase (SOD) and catalase (CAT) activity was observed when compared to the 1st HBO exposure; glutathione peroxidase (GPx) activity remained almost unchanged.Conclusions: In the absence of antioxidant supplementation, the prolonged HBO treatment leads to a condition of oxidative stress that seems to affect in particular the response of the enzymatic antioxidant defense system; the possible relationship between the chemical modifications of the enzymes caused by oxygen reactive species and the consequent inactivation of the proteins is under investigation. (C) 2004 The Canadian Society of Clinical Chemists. All rights reserved.