The optimum number and types of plasma samples necessary for an accurate activated partial thromboplastin time-based heparin therapeutic range.
The optimum number and types of plasma samples necessary for an accurate activated partial thromboplastin time-based heparin therapeutic range.
复制标题
准确的基于时间的活化部分凝血活酶肝素治疗范围所需的血浆样本的最佳数量和类型。
DOI：
10.5858/arpa.2011-0516-oa
复制
发表时间：
2013
影响因子：
4.6
通讯作者：
J. Gausman
中科院分区：
文献类型：
--
作者：
R. Marlar;J. Gausman
上下文
CONTEXT
Monitoring of unfractionated heparin therapy by activated partial thromboplastin time (aPTT) using the ex vivo method for determining the aPTT-based heparin therapeutic range (HTR) is the standard of practice. Many intrinsic and extrinsic factors influence its accuracy.
OBJECTIVE
To investigate the optimum number and types of samples acceptable for an accurate ex vivo HTR determination.
DESIGN
Values from patients receiving unfractionated heparin are used to determine the HTR by published guidelines. The number and types of samples are changed to investigate the effect on HTR parameters.
RESULTS
Absolute minimum number of samples for an accurate HTR is 20, with fewer than 10% of the samples from the same patient or 50% of the samples with international normalized ratio of 1.3 to 1.5.
CONCLUSIONS
The ex vivo HTR method is the best protocol currently available; however, the number of samples used affects its accuracy. The optimum number of samples is 30 or more but the absolute minimum number is 20. In addition, limitation of specific sample types also affects the HTR parameters. An inaccurate HTR may be calculated if inappropriate sample number or types of samples are used.