Fusarium graminearum gene deletion mutants map1 and tri5 reveal similarities and differences in the pathogenicity requirements to cause disease on Arabidopsis and wheat floral tissue
Fusarium graminearum gene deletion mutants map1 and tri5 reveal similarities and differences in the pathogenicity requirements to cause disease on Arabidopsis and wheat floral tissue
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DOI：
10.1111/j.1469-8137.2007.02333.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Hammond-Kosack, Kim
中科院分区：
文献类型：
--
作者：
Cuzick, Alayne;Urban, Martin;Hammond-Kosack, Kim
禾谷镰刀菌（Fusarium graminearum）是一种能侵染所有禾谷类作物的子囊菌，它能降低谷物的产量、品质和安全性。这种真菌也能在拟南芥上引起疾病。本研究对两株F.对禾谷镰刀菌野生型突变体进行了分析，以进一步探索小麦（Triticum aestivum）和拟南芥花病理系统之间的相似性。禾谷镰刀菌（菌株PH-1）和两个缺乏促分裂原活化蛋白激酶MAP 1基因或异戊二烯合酶TRI 5基因的同基因转化体分别喷雾或点接种到拟南芥和小麦花组织上。病害的发展进行了定量评估宏观和微观和脱氧雪腐镰刀菌烯醇（DON）真菌毒素浓度测定酶联免疫吸附测定（ELISA）。野生型菌株接种引起的高水平的疾病，在两种植物物种和显着的DON生产。map 1突变体在两种宿主中引起最小的疾病和DON积累。不能产生DON的tri 5突变体在小麦穗上表现出降低的致病性，仅在小穗上引起不连续的眼状病变，未能感染轴。与此相反，tri 5突变体对拟南芥花组织的致病性保持不变。graminearum定殖拟南芥花组织。
The Ascomycete pathogen Fusarium graminearum can infect all cereal species and lower grain yield, quality and safety. The fungus can also cause disease on Arabidopsis thaliana. In this study, the disease-causing ability of two F. graminearum mutants was analysed to further explore the parallels between the wheat (Triticum aestivum) and Arabidopsis floral pathosystems.Wild-type F. graminearum (strain PH-1) and two isogenic transformants lacking either the mitogen-activated protein kinase MAP1 gene or the trichodiene synthase TRI5 gene were individually spray- or point-inoculated onto Arabidopsis and wheat floral tissue. Disease development was quantitatively assessed both macroscopically and microscopically and deoxynivalenol (DON) mycotoxin concentrations determined by enzyme-linked immunosorbent assay (ELISA).Wild-type strain inoculations caused high levels of disease in both plant species and significant DON production. The map1 mutant caused minimal disease and DON accumulation in both hosts. The tri5 mutant, which is unable to produce DON, exhibited reduced pathogenicity on wheat ears, causing only discrete eye-shaped lesions on spikelets which failed to infect the rachis. By contrast, the tri5 mutant retained full pathogenicity on Arabidopsis floral tissue.This study reveals that DON mycotoxin production is not required for F. graminearum to colonize Arabidopsis floral tissue.