BACTERIAL-RESISTANCE TO COMPLEMENT KILLING MEDIATED BY THE AIL PROTEIN OF YERSINIA-ENTEROCOLITICA
BACTERIAL-RESISTANCE TO COMPLEMENT KILLING MEDIATED BY THE AIL PROTEIN OF YERSINIA-ENTEROCOLITICA
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DOI：
10.1073/pnas.89.8.3561
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发表时间：
1992-04-15
影响因子：
11.1
通讯作者：
FALKOW, S
中科院分区：
文献类型：
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作者：
BLISKA, JB;FALKOW, S
Ail 是一种 17 kDa 外膜耶尔森氏菌蛋白，介导细菌附着和侵袭培养的上皮细胞。我们在此报告了 Ail 在耶尔森氏菌感染发病机制中的另一种作用。我们发现，携带 4 KB 重组 ail 克隆 pVM102 的大肠杆菌 HB101 在高达 50% 的正常人血清中具有高度的杀灭能力。来自 pVM102 的 674 个碱基对的 DNA 片段（编码 ail 基因）被插入到 pUC18 中，并显示出可促进大肠杆菌 HB101 对补体杀伤的完全抵抗。质粒治愈的小肠结肠炎耶尔森氏菌(0:8)的inv菌株中的细胞附着和对补体杀伤的抗性与37℃下的Ail的热诱导表达相关。小肠结肠炎耶尔森氏菌中所有成分的插入灭活导致两种热诱导细菌特性的丧失。细胞附着和血清抗性通过质粒编码的所有缺陷的补充而得以恢复。细胞附着活性的补充需要细菌在 37 摄氏度下生长，这表明该 Ail 功能需要额外的热诱导因子。此外，这些研究表明Ail和结构相关蛋白Rck之间存在功能同源性，从而促进鼠伤寒沙门氏菌对补体杀伤的抵抗力。
Ail is a 17-kDa outer membrane Yersinia protein that mediates bacterial attachment to, and invasion of, cultured epithelial cells. We report here an alternative role for Ail in the pathogenesis of Yersinia infection. We found that Escherichia coli HB101 harboring the 4-kilobase recombinant ail clone pVM102 were highly resistant to killing in up to 50% normal human serum. A 674-base-pair fragment of DNA from pVM102, which encodes the ail gene, was inserted into pUC18 and shown to promote full resistance to complement killing in E. coli HB101. Cellular attachment and resistance to complement killing in a plasmid-cured inv- strain of Yersinia enterocolitica (0:8) was correlated with the thermoinduced expression of Ail at 37-degrees-C. Insertional inactivation of ail in Y. enterocolitica resulted in loss of both thermoinduced bacterial properties. Cellular attachment and serum resistance were restored by complementation of the defect by plasmid-encoded ail. Complementation of cell attachment activity required bacterial growth at 37-degrees-C, indicating that an additional thermoinduced factor is required for this Ail function. In addition, these studies reveal that functional homology exists between Ail and the structurally related protein Rck, which promotes resistance to complement killing in Salmonella typhimurium.