ISOLATION AND SEQUENCE OF A CDNA-ENCODING THE MAJOR STRUCTURAL PROTEIN OF PERIPHERAL MYELIN
ISOLATION AND SEQUENCE OF A CDNA-ENCODING THE MAJOR STRUCTURAL PROTEIN OF PERIPHERAL MYELIN
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DOI：
10.1016/0092-8674(85)90198-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
AXEL, R
中科院分区：
文献类型：
--
作者：
LEMKE, G;AXEL, R
髓鞘是神经系统特有的多层膜，其功能是作为绝缘体以大大增加轴突冲动传导的速度。差异筛选和杂交选择的技术被用来确定一个C[互补]DNA克隆编码[大鼠]雪旺细胞糖蛋白P0，主要结构蛋白的外周髓鞘。从克隆的cDNA的核苷酸序列推导出的该蛋白的序列表明，P0是一个完整的膜蛋白，包含一个单一的跨膜区，一个大的疏水性胞外结构域，和一个较小的基本胞内结构域。蛋白质的结构表明，这些结构域中的每一个在产生髓鞘的高度有序结构中起着重要作用。P0 mRNA的诱导与髓鞘形成的起始相一致，并提出了一个模型，其中糖蛋白作为这一过程的分子路标。
The myelin sheath is a multilayered membrane, unique to the nervous system, which functions as an insulator to increase greatly the velocity of axonal impulse conduction. The techniques of differential screening and hybrid selection were used to identify a c[complementary]DNA clone encoding the [rat] Schwann cell glycoprotein P0, the major structural protein of the peripheral myelin sheath. The sequence of this protein, deduced from the nucleotide sequence of the cloned cDNA, indicates that P0 is an integral membrane protein containing a single membrane-spanning region, a large hydrophobic extracellular domain, and a smaller basic intracellular domain. The structure of the protein suggests that each of these domains plays an essential role in generating the highly ordered structure of the myelin sheath. The induction of P0 mRNA coincides with the initiation of myelin formation, and a model is proposed in which the glycoprotein serves as a molecular guidepost for this process.