New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics
New tool for spreading proteins to the environment: Cry1Ab toxin immobilized to bioplastics
复制标题
将蛋白质传播到环境中的新工具：固定在生物塑料上的 Cry1Ab 毒素
DOI：
10.1007/s00253-005-0257-6
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发表时间：
2005
影响因子：
5
通讯作者：
M. A. Prieto
中科院分区：
文献类型：
--
作者：
Cristina Moldes;G. Farinós;L. I. Eugenio;P. García;J. García;F. Ortego;P. Hernández;P. Castanera;M. A. Prieto
基于聚羟基链烷酸酯（PHA，生物塑料）颗粒的使用，开发了一种新的工具，以提供一种环境友好的方式将活性蛋白质递送到环境中。为了说明这种新方法，衍生的Cry 1Ab昆虫特异性毒素蛋白通过多肽标签BioF在体内固定到PHA颗粒中。Fk-Bt 1是一种对Noctuidae（鳞翅目：夜蛾科）芝麻夜蛾（Sesamia nonagrioides）有较强杀虫活性的新型毒素。新的毒素颗粒制剂（PFk-Bt 1）和纯化的Cry 1Ab的剂量-死亡率反应进行了比较，证明了PFk-Bt 1的有效性，并提出了共同的作用模式。
A new tool to provide an environmentally friendly way to deliver active proteins to the environment has been developed, based on the use of polyhydroxyalkanoate (PHA, bioplastic) granules. To illustrate this novel approach, a derived Cry1Ab insect-specific toxin protein was in vivo immobilized into PHA granules through the polypeptide tag BioF. The new toxin, named Fk-Bt1, was shown to be active against Sesamia nonagrioides (Lepidoptera: Noctuidae). The dose–mortality responses of the new toxin granule formulation (PFk-Bt1) and purified Cry1Ab have been compared, demonstrating the effectiveness of PFk-Bt1 and suggesting a common mode of action.