Genetic diversity and relationships among Siberian and Far Eastern larches inferred from RAPD analysis
Genetic diversity and relationships among Siberian and Far Eastern larches inferred from RAPD analysis
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DOI：
10.1023/b:ruge.0000024978.25458.f7
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发表时间：
2004-04-01
影响因子：
0.6
通讯作者：
Zhuravlev, YN
中科院分区：
文献类型：
--
作者：
Kozyrenko, MM;Artyukova, EV;Zhuravlev, YN
对托木斯克、伊尔库茨克、乌兰乌德(西伯利亚)和布拉戈维申斯克、哈巴罗夫斯克、尤日诺-萨哈林斯克(远东)6个地理位置较远的落叶松品种进行了遗传多样性分析。用17个随机序列引物对457个随机扩增多态DNA基因座进行了比较，其中97%是多态的。在检测的样本中，32-49%的基因处于杂合态，平均期望杂合度(H-EXP)在0.1373-0.1891之间，不同样本对的遗传距离(D-N)在0.0361-0.1802之间。测定了兴安落叶松、兴安落叶松Larix siBirica Ledeb.、落叶松L.gmelinii(Rupr.)Rupr.和甘草L.kamtschatica(Rupr.)卡尔。亲缘关系分析表明，兴安落叶松的遗传分化程度最高，而兴安落叶松与西伯利亚落叶松的亲缘关系较近。
Genetic diversity of larches from six geographically distant regions, Tomsk, Irkutsk, Ulan-Ude (Siberia), and Blagoveshchensk, Khabarovsk, Yuzhno-Sakhalinsk (Far East) was examined by means of RAPD analysis. Tree DNA samples were compared using 457 RAPD loci (97% of which were polymorphic), identified with 17 primers of random sequences. In the samples examined, 32 to 49% of the genes were in heterozygous state, mean expected heterozygosity (H-exp) varied from 0.1373 to 0.1891, and the genetic distances (D-N) for different sample pairs varied from 0.0361 to 0.1802. The main population parameters were determined for Larix sibirica Ledeb., L. gmelinii (Rupr.) Rupr., and L. kamtschatica (Rupr.) Carr. Analysis of the genetic relationships showed that L. kamtschatica was characterized by highest genetic differentiation from the other larches examined, while larches from Primorskii krai were genetically close to L. sibirica.