Potential skin protective effects after UVB irradiation afforded by an antioxidant peptide from Odorrana andersonii
Potential skin protective effects after UVB irradiation afforded by an antioxidant peptide from Odorrana andersonii
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Odorrana andersonii 的抗氧化肽在 UVB 照射后具有潜在的皮肤保护作用
DOI：
10.1016/j.biopha.2019.109535
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发表时间：
2019-12-01
影响因子：
7.5
通讯作者：
Yang, Xinwang
中科院分区：
文献类型：
--
作者：
Yin, Saige;Wang, Ying;Yang, Xinwang
随着需求的增加，开发新的天然抗氧化剂已成为科学研究的主要方向。我们以前确定了一个短的基因编码的肽（OA-VI 12）从Odorrana andersonii青蛙皮肤分泌物，发挥直接清除自由基的能力，这表明可能的功能，在保护皮肤免受光损伤所造成的高海拔栖息地。在当前的研究中，我们检查了OA-VI 12对用人永生化角质形成细胞建立的UVB照射和过氧化氢诱导的氧化应激模型的影响。此外，我们还鉴定了氧化应激和OA-VI 12组中的差异表达基因（DEG），并进一步进行了转录组以及功能和途径富集分析。结果表明，OA-VI 12保护细胞活力，促进过氧化氢酶的释放，降低乳酸脱氢酶和活性氧的水平。此外，该肽促进超氧化物歧化酶和谷胱甘肽的产生，减轻表皮和真皮厚度，并减少光损伤小鼠模型皮肤中光点和胶原纤维的产生。京都基因百科全书和基因组分析表明，丝裂原活化蛋白激酶（MAPK）是最丰富的信号通路。基因本体（GO）分析表明，前55个显着丰富的GO术语主要涉及细胞的过程，部分和结合。这些结果揭示了小分子基因编码的抗氧化肽（OA-VI 12）的有益作用及其作为光损伤保护剂的潜在应用。
With increasing demand, the development of new natural antioxidants has become a primary direction of scientific research. We previously identified a short gene-encoded peptide (OA-VI12) from Odorrana andersonii frog skin secretions that exerted direct scavenging capacity against free radicals, suggesting a possible function in protecting skin against photodamage caused by their high-altitude habitat. In the current research, we examined the effects of OA-VI12 on both UVB-irradiation and hydrogen peroxide-induced oxidative stress models established with human immortalized keratinocytes. In addition, we identified the differentially expressed genes (DEGs) in the oxidative stress and OA-VI12 groups and further performed transcriptome as well as functional and pathway enrichment analyses. Results showed that OA-VI12 protected cell viability, promoted the release of catalase, and decreased the levels of lactate dehydrogenase and reactive oxygen species. Moreover, the peptide promoted the production of superoxide dismutase and glutathione, alleviated epidermis and dermis thickness, and decreased the production of light spots and collagen fibers in skin from the photo-injured mouse model. Kyoto Encyclopedia of Genes and Genomes analysis showed mitogen-activated protein kinase (MAPK) to be the most abundant signaling pathway. Gene Ontology (GO) analysis indicated that the top 55 significantly enriched GO terms mainly involved cellular processes, parts, and binding. These results revealed the beneficial role of the small molecule gene-encoding antioxidant peptide (OA-VI12) and its potential application as a protective agent against photodamage.