Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen).
Particle-mediated delivery of recombinant expression vectors to rabbit skin induces high-titered polyclonal antisera (and circumvents purification of a protein immunogen).
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颗粒介导的重组表达载体递送至兔皮肤可诱导高滴度的多克隆抗血清（并避免蛋白质免疫原的纯化）。
DOI：
10.1093/nar/24.7.1375
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发表时间：
1996
影响因子：
14.9
通讯作者：
Brandsma,JL
中科院分区：
文献类型：
--
作者：
Sundaram,P;Xiao,W;Brandsma,JL
通过将包被有编码细胞质（β-半乳糖苷酶）或核（棉尾兔乳头瘤病毒的L1衣壳）蛋白的哺乳动物表达载体的金颗粒递送至皮肤，在兔中产生多克隆抗体。用30 μg DNA/只兔进行一次初次免疫和一次加强免疫，在8只兔中每只兔产生的特异性抗血清的滴度为1：24 000至1：120 000，通过ELISA和Western印迹分析检测。遗传免疫需要相对少量的DNA，消除了纯化蛋白免疫原的需要，并且不需要刺激性佐剂。
Polyclonal antibodies were generated in rabbits by delivery to skin of gold particles coated with mammalian expression vectors encoding a cytoplasmic (β-galactosidase) or a nuclear (L1 capsid of cottontail rabbit papillomavirus) protein. One primary and one booster immunization of 30 µg DNA per rabbit yielded specific antisera with titers from 1:24 000 to 1:120 000 in each of eight rabbits, as detected by ELISA and Western blot analysis. Genetic immunization requires relatively small amounts of DNA, eliminates the need to purify the protein immunogen, and does not require irritating adjuvants.