There is more than one kind of myofibroblast: analysis of CD34 expression in benign, in situ, and invasive breast lesions
There is more than one kind of myofibroblast: analysis of CD34 expression in benign, in situ, and invasive breast lesions
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DOI：
10.1136/jcp.56.4.271
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发表时间：
2003-04-01
影响因子：
3.4
通讯作者：
Jones, JL
中科院分区：
文献类型：
--
作者：
Chauhan, H;Abraham, A;Jones, JL
目的：平滑肌肌动蛋白（SMA）阳性的肌成纤维细胞与肿瘤侵袭有关；然而，SMA的获得并不局限于肿瘤周围的成纤维细胞，成纤维细胞的其他变化可能与恶性环境更具体地相关。CD34是一种正常乳腺成纤维细胞表达的唾液黏液蛋白，但在浸润性癌中缺失。本研究的目的是建立乳腺成纤维细胞中CD34与SMA表达之间的关系，并分析CD34的缺失是否对侵袭性疾病具有特异性。方法：对135例患者进行CD34和SMA免疫组化检测，其中正常10例，纤维腺瘤10例，浸润性导管癌40例，导管原位癌55例，放射状瘢痕/复杂硬化病变20例。染色模式与组织病理学特征之间的关系记录为阳性、阴性或减弱。结果：正常导管小叶周围及上皮增生的成纤维细胞CD34阳性，以SMA阴性为主。在纤维腺瘤中，成纤维细胞保留CD34，但获得SMA表达。浸润性癌周围成纤维细胞CD34阳性，SMA阳性。负的。在DCIS中，CD34的丢失在高级别肿瘤中比在低级别或中级别肿瘤中更为常见
Aims: Smooth muscle actin (SMA) positive myofibroblasts have been implicated in tumour invasion; however, acquisition of SMA is not limited to peritumorous fibroblasts and other changes in fibroblasts may be more specifically related to the malignant environment. CD34 is a sialomucin expressed by normal breast fibroblasts but lost in invasive carcinomas. The aim of this study was to establish the relation between CD34 and SMA expression in breast fibroblasts and to analyse whether loss of CD34 is specific for invasive disease.Methods: Immunohistochemistry for CD34 and SMA was performed on 135 cases including 10 normal, 10 fibroadenomas, 40 infiltrating ductal carcinomas, 55 cases of ductal carcinoma in situ (DCIS), and 20 radial scar/complex sclerosing lesions. The relation between staining pattern and histopathological features was recorded as positive, negative, or reduced.Results: Fibroblasts around all normal duct-lobule units and those showing epithelial hyperplasia were CD34 positive and mainly SMA negative. In fibroadenomas, fibroblasts retained CD34 but acquired SMA expression. In contrast, fibroblasts around invasive carcinoma were CD34 positive and SMA. negative. In DCIS, loss of CD34 was significantly more frequent in high grade tumours than in low or intermediate grade ones (p