Sonodynamically-induced cytotoxicity by rose bengal derivative and microbubbles in isolated sarcoma 180 cells
Sonodynamically-induced cytotoxicity by rose bengal derivative and microbubbles in isolated sarcoma 180 cells
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DOI：
10.7567/jjap.54.07hf16
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发表时间：
2015-06
影响因子：
1.5
通讯作者：
N. Sugita;M. Hosokawa;Naoki Sunaga;Y. Iwase;N. Yumita;T. Ikeda;S. Umemura
中科院分区：
文献类型：
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作者：
N. Sugita;M. Hosokawa;Naoki Sunaga;Y. Iwase;N. Yumita;T. Ikeda;S. Umemura
超声与声动力增敏剂（SDS）联合应用是一种有效的非侵入性肿瘤治疗方法，称为声动力疗法（SDT）。微泡也已用于超声治疗。本文的目的是阐明微泡对SDT的影响。将肉瘤180细胞悬浮在空气饱和磷酸盐缓冲盐水中，并在存在和不存在微泡[Sonazoid（SZ）]的情况下，以驻波模式暴露于SDS虎红衍生物（RBD）超声。超声诱导的细胞毒性与RBD和SZ是约20倍，比没有，约80%的SZ微泡被破坏的超声暴露在短短5秒。由于即使在短时间、低强度的超声下，微泡也会诱导显著的细胞毒性，因此微泡在SDT中的应用显示出抗肿瘤治疗的前景。
It is known that the combination of ultrasound and sonodynamic sensitizer (SDS) is effective in noninvasive tumor treatment, referred to as sonodynamic therapy (SDT). Microbubbles have been used in ultrasound therapy as well. The purpose of this paper is to clarify the effect of microbubbles on SDT. Sarcoma 180 cells were suspended in air-saturated phosphate-buffered saline and exposed to ultrasound with the SDS rose bengal derivative (RBD) in standing wave mode in the presence and absence of microbubbles [sonazoid (SZ)]. The ultrasonically induced cytotoxicity with RBD and SZ was about 20 times higher than without either, and about 80% of the SZ microbubbles were destructed by ultrasonic exposure in as short as five seconds. Since microbubbles induce significant cytotoxicity even with short duration, low intensity ultrasound, the application of microbubbles in SDT shows promise in anti-tumor treatment.