ANOMALOUS TEMPERATURE FACTOR BEHAVIOR AND CRYSTAL-LATTICE MOBILITY IN CYTOCHROME-C'

ANOMALOUS TEMPERATURE FACTOR BEHAVIOR AND CRYSTAL-LATTICE MOBILITY IN CYTOCHROME-C'
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DOI:
10.1016/s0006-3495(86)83598-6
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发表时间:
1986-01-01
影响因子:
3.4
通讯作者:
SALEMME, FR
SALEMME, FR
中科院分区:
生物学3区
文献类型:
--
作者:
FINZEL, BC;SALEMME, FR

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导致在低压下失活的缔合和解离的重复循环也可以在大气压和低温下发生。混合 H4 和 M4 (7, uM) 的杂交量在冰箱 (4.30 C) 中放置 6 天后发生,并且该数量在 12 天内翻倍。图3显示了冷样品在室温下保存后活性随时间恢复的过程。恢复完全活动需要几个小时,恢复时间取决于浓度;浓度越高恢复越快。这一观察结果与
The repeated cycles of association and dissociation that lead to inactivation at low pressures can be shown to take place also at atmospheric pressure at low temperature. Hybridization of-26% of mixed H4 and M4 (7, uM) takes place after six days in the refrigerator (4.30 C) and this amount doubles in 12 d. Fig. 3 shows the course of the activity recovery with time after the cold samples are stored at room temperature. It takes several hours for the return of full activity and the time for recovery is concen-tration-dependent; faster recoveryoccurs at the higher concentrations. This observation is fully consistent with the