A simplified method for studies of haemoglobin biosynthesis.
A simplified method for studies of haemoglobin biosynthesis.
复制标题
血红蛋白生物合成研究的简化方法。
DOI：
10.1111/j.1365-2257.1980.tb00824.x
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Ernest Beutler
中科院分区：
文献类型：
--
作者：
C. Tegos;Ernest Beutler
以35S-蛋氨酸为标记氨基酸，用凝胶电泳法分离球蛋白链，建立了研究血红蛋白链生物合成的简化方法。这项技术简化了珠蛋白的制备过程，改善了珠蛋白链的电泳分离，并可应用于网织红细胞计数正常的样品。可以同时制备多达20个样品，一次可以对10个样品进行电泳分离。该方法用于外周血或骨髓的正常和异常样本时，可获得预期的合成比率，并具有很高的重复性。
A simplified method for studies of haemoglobin chain biosynthesis using 35S-methionine as the labeled amino acid and Cellogel electrophoresis for globin chain separation is described. This technique simplifies the procedure globin preparation, improves the electrophoretic separation of globin chains and may be applied to samples with normal reticulocyte counts. As many as 20 samples can be prepared simultaneously and 10 samples can be subjected to electrophoretic separation at one time. The method was fund to yield the expected synthetic ratios and to be highly reproducible when applied to normal and abnormal samples from peripheral blood or bone marrow.