MASP-2 activation is involved in ischemia-related necrotic myocardial injury in humans.
MASP-2 activation is involved in ischemia-related necrotic myocardial injury in humans.
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MASP-2 激活参与人类缺血相关的坏死性心肌损伤。
DOI：
10.1016/j.ijcard.2011.11.032
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发表时间：
2013
影响因子：
3.5
通讯作者：
Shevde,Ketan
中科院分区：
文献类型：
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作者：
Zhang,Ming;Hou,YunfangJoan;Cavusoglu,Erdal;Lee,DanielC;Steffensen,Rudi;Yang,Liming;Bashari,Daniel;Villamil,Jose;Moussa,Motaz;Fernaine,George;Jensenius,JensC;Marmur,JonathanD;Ko,Wilson;Shevde,Ketan
背景/目的心脏血液供应不足会导致缺血性损伤，临床表现为心肌梗死（MI）。缺血后，炎症被激发并与临床结果相关。最近的基础科学研究表明，补体因子MASP-2在缺血/再灌注损伤的动物模型中起重要作用。我们在两种临床环境中研究了MASP-2在人急性心肌缺血中的作用：（1）急性MI，和（2）心脏直视手术。方法：总共187名人类受试者参加了这项研究，包括50名健康个体，27名被诊断为冠状动脉疾病（CAD）但没有急性MI的患者，29名被转诊进行冠状动脉造影的急性MI患者，81例心脏手术后出现急性心肌缺血的患者。通过ELISA测量循环MASP-2水平。与健康个体或无急性MI的CAD患者相比，MI患者外周循环中的MASP-2水平显著降低。在接受手术诱导的全心缺血的心脏病患者中评估了急性心肌缺血期间MASP-2被激活的假设。发现MASP-2在这些患者的冠状动脉循环中显著降低，并且MASP-2水平的降低与心肌坏死标志物心肌肌钙蛋白I的增加独立相关。结论这些结果表明MASP-2参与人类缺血相关的坏死性心肌损伤。
BACKGROUND/OBJECTIVESInsufficient blood supply to the heart results in ischemic injury manifested clinically as myocardial infarction (MI). Following ischemia, inflammation is provoked and related to the clinical outcomes. A recent basic science study indicates that complement factor MASP-2 plays an important role in animal models of ischemia/reperfusion injury. We investigated the role of MASP-2 in human acute myocardial ischemia in two clinical settings: (1) Acute MI, and (2) Open heart surgery.METHODSA total of 187 human subjects were enrolled in this study, including 50 healthy individuals, 27 patients who were diagnosed of coronary artery disease (CAD) but without acute MI, 29 patients with acute MI referred for coronary angiography, and 81 cardiac surgery patients with surgically-induced global heart ischemia. Circulating MASP-2 levels were measured by ELISA.RESULTSMASP-2 levels in the peripheral circulation were significantly reduced in MI patients compared with those of healthy individuals or of CAD patients without acute MI. The hypothesis that MASP-2 was activated during acute myocardial ischemia was evaluated in cardiac patients undergoing surgically-induced global heart ischemia. MASP-2 was found to be significantly reduced in the coronary circulation of such patients, and the reduction of MASP-2 levels correlated independently with the increase of the myocardial necrosis marker, cardiac troponin I.CONCLUSIONSThese results indicate an involvement of MASP-2 in ischemia-related necrotic myocardial injury in humans.