CHANGES IN THE DISTRIBUTION OF MICROTUBULES AND INTERMEDIATE FILAMENTS IN MAMMALIAN SERTOLI CELLS DURING SPERMATOGENESIS
CHANGES IN THE DISTRIBUTION OF MICROTUBULES AND INTERMEDIATE FILAMENTS IN MAMMALIAN SERTOLI CELLS DURING SPERMATOGENESIS
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DOI：
10.1002/ar.1092200206
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发表时间：
1988-02-01
影响因子：
--
通讯作者：
VOGL, AW
中科院分区：
文献类型：
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作者：
AMLANI, S;VOGL, AW
本文研究了哺乳动物睾丸支持细胞在精子发生过程中微管和中间丝的分布。通过间接免疫荧光法测定地松鼠睾丸中微管的排列，所述地松鼠睾丸1）固定，机械破碎，并附着于聚赖氨酸包被的载玻片，和2）固定，包埋在聚乙二醇中，并切片。在未固定的大鼠睾丸切片中，还通过间接免疫荧光法测定了中间丝模式。这些研究的结果得到了证实，并使用电子显微镜进行了扩展。微管首先在包围圆形精子细胞的侧突中变得明显。当精子细胞伸长并位于支持细胞的顶隐窝时，微管变得平行于支持细胞的长轴并围绕隐窝。随着精子细胞成熟并获得碟形，顶端微管逐渐集中在顶体附近的支持细胞区域，最终形成离散的C形结构，在精子形成过程中消失。大鼠支持细胞的中间纤维以细胞核为中心。从核周区，细丝延伸到桥粒样结与早期生精细胞和顶端的细胞质，在那里他们有一个短暂的协会与隐窝含有细长的精子细胞。在精子发生周期的早期阶段，当顶隐窝位于上皮深处时，隐窝中的细丝最明显。当精子细胞位于更顶端的位置时，它们变得不那么明显并最终消失。我们的荧光研究和超微结构分析表明，协会的中间丝与隐窝是特定的区域相邻的精子细胞头的背面或凸方面。在这些区域中，大约8至12个均匀排列的细丝与围绕隐窝的外质特化中的肌动蛋白细丝密切相关。我们的结论是，像肌动蛋白，微管和中间丝的分布变化在支持细胞在精子发生过程中。微管的分布与支持细胞的不规则柱状有关。我们怀疑，位于顶端的中间丝可能发挥作用，在固定或定位支持细胞隐窝深处的上皮在生精周期的早期阶段。
We have studied the distribution of microtubules and intermediate filaments in mammalian Sertoli cells during spermatogenesis. The arrangement of microtubules was determined, by indirect immunofluorescence, in ground squirrel testes that were 1) fixed, mechanically fragmented, and attached to polylysine-coated slides, and 2) fixed, embedded in polyethylene glycol, and sectioned. Intermediate filament patterns were determined, also by indirect immunofluorescence, in sections of unfixed rat testis. Results from these studies were confirmed and extended using electron microscopy. Microtubules first become evident in lateral processes that embrace round spermatids. When spermatids elongate and become situated in apical crypts of Sertoli cells, the microtubules become oriented parallel to the long axis of Sertoli cells and surround the crypts. As spermatids mature and acquire a saucer shape, apical microtubules progressively concentrate in Sertoli cell regions adjacent to the acrosome and eventually form discrete C-shaped structures that disappear during spermiation. Intermediate filaments in rat Sertoli cells are centered around the nucleus. From perinuclear regions, filaments extend toward desmosome-like junctions with early spermatogenic cells and into the apical cytoplasm where they have a transient association with crypts containing elongate spermatids. Filaments amongst crypts are most evident in early stages of the spermatogenic cycle when apical crypts are situated deep within the epithelium. They become less evident and eventually disappear as spermatids assume a more apical position. Our fluorescence studies and ultrastructural analyses indicate that the association of intermediate filaments with crypts is specific to regions adjacent to the dorsal or convex aspect of spermatid heads. In these regions, approximately 8 to 12 uniformly aligned filaments are intimately associated with actin filaments in ectoplasmic specializations surrounding the crypts. We conclude that, like actin, the distribution of microtubules and intermediate filaments changes in Sertoli cells during spermatogenesis. The distribution of microtubules correlates with the irregular columnar shape of Sertoli cells. We suspect that the apically situated intermediate filaments may play a role in anchoring or positioning Sertoli cell crypts deep within the epithelium during the early stages of the spermatogenic cycle.