Biochip System for Rapid and Accurate Identification of Mycobacterial Species from Isolates and Sputum
Biochip System for Rapid and Accurate Identification of Mycobacterial Species from Isolates and Sputum
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DOI：
10.1128/jcm.00158-10
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发表时间：
2010-10-01
影响因子：
9.4
通讯作者：
Guo, Yong
中科院分区：
文献类型：
--
作者：
Zhu, Lingxiang;Jiang, Guanglu;Guo, Yong
从分离株和临床标本中准确检测分枝杆菌种类对病原诊断、治疗和疾病控制具有重要意义。目前迫切需要开发一种快速、简便、准确的检测方法。我们建立了一个生物芯片检测系统，包括生物芯片、样品制备装置、杂交仪器、芯片洗衣机和配备自动诊断解释软件的激光共聚焦扫描仪。该生物芯片通过定位16S rRNA的差异，同时鉴定出17种常见的分枝杆菌。采用64株参考菌株、296株结核分枝杆菌和243株非结核分枝杆菌、138株其他细菌和195份痰液样本对系统进行评估，并进行DNA测序比较。整个生物芯片检测耗时6 h。生物芯片检测结果与DNA测序结果的一致性为100%。总之，该生物芯片系统提供了一种简单、快速、可靠和高度准确的临床检测方法，可在6小时内从培养分离物或痰样本中测定分枝杆菌种类，从而允许对患者进行早期病原体适应的抗菌治疗。
The accurate detection of mycobacterial species from isolates and clinical samples is important for pathogenic diagnosis and treatment and for disease control. There is an urgent need for the development of a rapid, simple, and accurate detection method. We established a biochip assay system, including a biochip, sample preparation apparatus, hybridization instrument, chip washing machine, and laser confocal scanner equipped with interpretation software for automatic diagnosis. The biochip simultaneously identified 17 common mycobacterial species by targeting the differences in the 16S rRNA. The system was assessed with 64 reference strains and 296 Mycobacterium tuberculosis and 243 nontuberculous mycobacterial isolates, as well as 138 other bacteria and 195 sputum samples, and then compared to DNA sequencing. The entire biochip assay took 6 h. The concordance rate between the biochip assay and the DNA sequencing results was 100%. In conclusion, the biochip system provides a simple, rapid, reliable, and highly accurate clinical assay for determination of mycobacterial species in a 6-h procedure, from either culture isolates or sputum samples, allowing earlier pathogen-adapted antimicrobial therapy in patients.