Proangiogenic potential of a collagen/bioactive glass substrate
Proangiogenic potential of a collagen/bioactive glass substrate
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DOI：
10.1007/s11095-007-9508-9
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发表时间：
2008-05-01
影响因子：
3.7
通讯作者：
Leach, J. Kent
中科院分区：
文献类型：
--
作者：
Leu, Ann;Leach, J. Kent
目的：先前刺激血管生成的尝试主要集中在生长因子和细胞因子的传递，编码特定血管生成诱导蛋白或转录因子的基因，或参与细胞。虽然高浓度的生物活性玻璃具有成骨潜力，但最近的研究表明，低浓度的特定生物活性玻璃具有血管生成作用。我们假设一种已知的生物活性玻璃（bioslass (R) 45S5）在有限的浓度范围内具有促进血管生成的潜力。材料和方法：将不同数量的生物玻璃装入可吸收的胶原海绵中。生物玻璃的促血管生成潜能是通过检测其可溶性产物诱导内皮细胞增殖、共培养中小管形成和上调血管内皮生长因子（VEGF）产生的能力来确定的。结果：我们确定了一系列具有促血管生成潜力的生物玻璃浓度。此外，我们证明了这种材料的促血管生成能力与生物玻璃的可溶性溶解产物以及受刺激细胞随后产生的细胞分泌的血管生成因子有关。结论：这些研究表明，这种生物活性玻璃具有强大的促血管生成潜力，这种策略可能提供重组诱导生长因子的替代方案。
Purpose: Previous attempts to stimulate angiogenesis have focused on the delivery of growth factors and cytokines, genes encoding for specific angiogenic inductive proteins or transcription factors, or participating cells. While high concentrations of bioactive glasses have exhibited osteogenic potential, recent studies have demonstrated that low concentrations of particular bioactive glasses are angiogenic. We hypothesized that a well known bioactive glass (Bioglass(R) 45S5) possesses proangiogenic potential over a limited range of concentrations.Materials and Methods: Varying amounts of Bioglass were loaded into absorbable collagen sponges. The proangiogenic potential of Bioglass was determined by examining the capacity of the soluble products to induce endothelial cell proliferation, tubule formation in a co-culture, and upregulate vascular endothelial growth factor (VEGF) production.Results: We determined a range of Bioglass concentrations which exhibit proangiogenic potential. Furthermore, we demonstrated that the proangiogenic capacity of this material is related to the soluble dissolution products of Bioglass and the subsequent production of cell-secreted angiogenic factors by stimulated cells.Conclusions: These studies suggest that this bioactive glass possesses a robust proangiogenic potential, and this strategy may provide an alternative to recombinant inductive growth factors.