Inactivation of Escherichia coli glycerol kinase by 5'-[p-(fluorosulfonyl)benzoyl]adenosine: protection by the hydrolyzed reagent.
Inactivation of Escherichia coli glycerol kinase by 5'-[p-(fluorosulfonyl)benzoyl]adenosine: protection by the hydrolyzed reagent.
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5-[p-(氟磺酰基)苯甲酰基]腺苷灭活大肠杆菌甘油激酶：水解试剂的保护。
DOI：
10.1021/bi00380a035
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Pettigrew,DW
中科院分区：
文献类型：
--
作者：
Pettigrew,DW
材料和方法材料。所有化学品和酶均购自西格玛化学公司，除非另有说明。放射性标记FS02BzAdo由Wyatt和Colman(1977)用ICN制药公司的[2，8-~3H]腺苷合成。六甲基磷三胺、腺苷、对-(氟磺酰基)苯甲酰氯、丙烷-1，2-二醇和丙烷-1，3-二醇由Aldrich化学公司的硅胶板提纯而成。
Materials and MethodsMaterials. All chemicals and enzymes were purchased from Sigma Chemical Co., unless indicated otherwise. Radioactively labeled FS02BzAdo was synthesized as described by Wyatt and Colman (1977) with [2, 8-3H] adenosine, which was purchased from ICN Pharmaceuticals, Inc. Hexamethylphosphoric triamide, adenosine, p-(fluorosulfonyl) benzoyl chloride, propane-1, 2-diol, and propane-1, 3-diol were pur-chased from Aldrich Chemical Co. Dimethylformamide was a product of Pierce Chemical Co. Silica gel plates with