Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study.
Age-related changes in gut microbiota composition from newborn to centenarian: a cross-sectional study.
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肠道菌群组成的年龄相关变化从新生儿到百年纪念：一项横断面研究。
DOI：
10.1186/s12866-016-0708-5
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发表时间：
2016-05-25
期刊：
影响因子：
4.2
通讯作者：
Osawa R
中科院分区：
文献类型：
--
作者：
Odamaki T;Kato K;Sugahara H;Hashikura N;Takahashi S;Xiao JZ;Abe F;Osawa R
据报道，人类肠道菌群的组成随着年龄的增长而变化；然而，很少有研究使用分子技术来研究肠道微生物群组成的长期、连续变化。在这项研究中，我们调查了新生儿到百岁日本受试者肠道微生物群组成的顺序变化。采用16S rRNA基因V3-V4区扩增子的高通量测序方法，对367名年龄在0 ~ 104岁之间的健康日本人的粪便样本进行了分析。基于肯德尔相关性定义的细菌共丰度群（CAGs）分析显示，婴儿、成人、老年人以及婴幼儿和老年人受试者中都富含某些过渡类型的微生物群。与婴儿和成人相关的cag相比，在老年相关的cag中观察到的相对丰度之间的正相关关系。基于属丰度的分层Ward连锁聚类分为5个聚类，年龄中位数（四分位间距）分别为3岁（0-35岁）、33岁（24-45岁）、42岁（32-62岁）、77岁（36-84岁）和94岁（86-98岁）。受试者主要与他们的年龄相匹配；然而，他们中的一些人属于年龄不匹配的群体。此外，基于基于群落系统发育调查（PICRUSt）预测的转运蛋白比例的聚类结果显示，受试者被分为两个与年龄相关的组，即成人富集组和婴儿/老年人富集组。值得注意的是，所有基于京都基因与基因组百科全书（KEGG）同源群的药物转运蛋白都出现在婴儿/老年人富集的聚类中。我们的研究结果表明，随着年龄的增长，肠道微生物群的组成变化存在一些模式和过渡点。此外，转运体特性预测结果表明，肠道中的营养物质可能在随着年龄变化的肠道微生物群组成中发挥重要作用。本文的在线版本（doi:10.1186/s12866-016-0708-5）包含补充材料，可供授权用户使用。
It has been reported that the composition of human gut microbiota changes with age; however, few studies have used molecular techniques to investigate the long-term, sequential changes in gut microbiota composition. In this study, we investigated the sequential changes in gut microbiota composition in newborn to centenarian Japanese subjects. Fecal samples from 367 healthy Japanese subjects between the ages of 0 and 104 years were analyzed by high-throughput sequencing of amplicons derived from the V3-V4 region of the 16S rRNA gene. Analysis based on bacterial co-abundance groups (CAGs) defined by Kendall correlations between genera revealed that certain transition types of microbiota were enriched in infants, adults, elderly individuals and both infant and elderly subjects. More positive correlations between the relative abundances of genera were observed in the elderly-associated CAGs compared with the infant- and adult-associated CAGs. Hierarchical Ward’s linkage clustering based on the abundance of genera indicated five clusters, with median (interquartile range) ages of 3 (0–35), 33 (24–45), 42 (32–62), 77 (36–84) and 94 (86–98) years. Subjects were predominantly clustered with their matched age; however, some of them fell into mismatched age clusters. Furthermore, clustering based on the proportion of transporters predicted by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) showed that subjects were divided into two age-related groups, the adult-enriched and infant/elderly-enriched clusters. Notably, all the drug transporters based on Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology groups were found in the infant/elderly-enriched cluster. Our results indicate some patterns and transition points in the compositional changes in gut microbiota with age. In addition, the transporter property prediction results suggest that nutrients in the gut might play an important role in changing the gut microbiota composition with age. The online version of this article (doi:10.1186/s12866-016-0708-5) contains supplementary material, which is available to authorized users.