Regulatable endogenous production of cytokinins up to 'toxic' levels in transgenic plants and plant tissues.
Regulatable endogenous production of cytokinins up to 'toxic' levels in transgenic plants and plant tissues.
复制标题
在转基因植物和植物组织中可调节细胞分裂素的内源产生至“有毒”水平。
DOI：
10.1007/bf00038992
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发表时间：
1993
影响因子：
5.1
通讯作者：
Key,JL
中科院分区：
文献类型：
--
作者：
Ainley,WM;McNeil,KJ;Hill,JW;Lingle,WL;Simpson,RB;Brenner,ML;Nagao,RT;Key,JL
在转基因烟草植株中，分析了表达由大豆热休克基因启动子和编码酶的序列组成的嵌合基因的效果，该酶催化合成一种有效的植物激素--细胞分裂素IPMP。细胞分裂素的产生内源性地产生了几种以前没有文献记载的影响。不依赖于外源细胞分裂素的转基因植物叶片经热处理后分化出的芽表明，长期的热处理不干扰复杂的发育过程。这扩大了热休克基因启动子的潜在用途，在跨越几周的发育窗口中有条件地表达基因。
The effects of expressing a chimeric gene consisting of a soybean heat shock gene promoter and a sequence that encodes an enzyme catalyzing the synthesis of a potent phytohormone, the cytokinin iPMP, have been analyzed in transgenic tobacco plants. The production of cytokinin endogenously produced several effects previously undocumented. The differentiation of shoots independent of exogenous cytokinin from heat-treated transgenic plant leaf explants demonstrates that long-term heat treatments do not interfere with complex developmental processes. This extends the potential usefulness of heat shock gene promoters to conditionally express genes during windows of development that span several weeks.