Identification of a novel type C porcine endogenous retrovirus: evidence that copy number of endogenous retroviruses increases during host inbreeding
Identification of a novel type C porcine endogenous retrovirus: evidence that copy number of endogenous retroviruses increases during host inbreeding
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DOI：
10.1099/0022-1317-82-8-1829
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发表时间：
2001-08-01
影响因子：
3.8
通讯作者：
van der Kuyl, AC
中科院分区：
文献类型：
--
作者：
Mang, R;Maas, J;van der Kuyl, AC
从猪基因组中分离出不同类别的猪内源性逆转录病毒(PERV)，它们可能在异种移植期间感染人类。由于脊椎动物基因组可能包含大量内源性逆转录病毒序列，因此对猪基因组进行了检查，以寻找其他内源性逆转录病毒，从而分离出一种新的、完整的内源性逆转录病毒基因组，命名为PERV-E。PERV-E的gag、pol和env基因与属于HERV-E家族的人内源性逆转录病毒(HERV)4-1的GAG、POL和env基因密切相关。确定PERV的存在和拷贝数的研究结果表明，在所有被测基因组中都存在PERV-E和PERV-A/B样前病毒，但在包括野猪在内的两个被研究物种中没有发现PERV-C。在每个猪基因组中都可以发现PERV的多个拷贝。在所有被测试的猪基因组中，野猪基因组的拷贝数是所有PERV中最低的，这表明近亲繁殖增加了完整内源性逆转录病毒的整合数量。
Different classes of porcine endogenous retroviruses (PERVs), which have the potential to infect humans during xenotransplantation, have been isolated from the pig genome. Because vertebrate genomes may contain numerous endogenous retrovirus sequences, the pig genome was examined for additional endogenous retroviruses, resulting in the isolation of a novel, complete endogenous retrovirus genome, designated PERV-E. The gag, pol and env genes of PERV-E are closely related to those of human endogenous retrovirus (HERV) 4-1, which belongs to the HERV-E family. Results of stud ies to determine the presence and copy number of PERVs demonstrated that PERV-E and PERV-A/B-like proviruses were present in all genomes tested, but that PERV-C was not found in two of the species examined, including wild boar. Multiple copies of PERVs could be found in each pig genome. Among all of the pig genomes tested, the wild boar genome had the lowest copy number of all PERVs, suggesting that the number of integrations of complete endogenous retroviruses is increased by inbreeding.