The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing
The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing
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DOI：
10.1002/term.2691
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发表时间：
2018-06-01
影响因子：
3.3
通讯作者：
Man, Yi
中科院分区：
文献类型：
--
作者：
Qu, Yili;Cao, Cong;Man, Yi
与皮肤再生相关的主要挑战可包括血管化受阻和上皮化程度不足。鉴于这些过程的复杂性和它们所依赖的控制信号，这些限制的一种可能的解决方案可以是通过多种细胞因子的外源性递送来模拟正常皮肤发育和伤口修复。在这里，我们报告使用角质细胞生长因子（KGF或FGF-7）和碱性成纤维细胞生长因子（bFGF或FGF-2）释放化学修饰的胶原蛋白膜，以促进皮肤伤口愈合。体外研究的结果证实，该系统导致更高的细胞增殖和更快的细胞迁移。将生物材料移植到切除创面愈合模型上后，与单一生长因子组和空白对照组相比，双生长因子组显示出更高度发达的血管网络和更有组织的表皮再生。因此，该实验组显示出成熟的表皮覆盖。总的来说，这种从胶原蛋白膜释放生长因子的新方法为满足伤口护理的未满足的临床需求开辟了新途径。
The major challenges associated with skin regeneration can include hindered vascularization and an insufficient degree of epithelization. In view of the complexity of these processes and the control signals on which they depend, one possible solution to these limitations could be simulating normal skin development and wound repair via the exogenous delivery of multiple cytokines. Here, we report the use of keratinocyte growth factor (KGF or FGF-7) and basic fibroblast growth factor (bFGF or FGF-2) released chemically modified collagen membranes to facilitate skin wound healing. The results from in vitro studies confirmed that this system resulted in higher cellular proliferation and faster cell migration. After transplanting the biomaterial onto an excisional wound healing model, the dual growth factor group, compared with the single growth factor groups and empty control group, showed more highly developed vascular networks and organized epidermal regeneration in the wounds. As a consequence, this experimental group showed mature epidermal coverage. Overall, this novel approach of releasing growth factors from a collagen membrane opens new avenues for fulfilling unmet clinical needs for wound care.