Prevalence of ESBL-producing Escherichia coli and carbapenem-resistant Enterobacteriaceae in treated wastewater: a comparison with nosocomial infection surveillance
Prevalence of ESBL-producing Escherichia coli and carbapenem-resistant Enterobacteriaceae in treated wastewater: a comparison with nosocomial infection surveillance
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DOI：
10.2166/wh.2020.014
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发表时间：
2020-12-01
影响因子：
2.3
通讯作者：
Tsutsui, Hirofumi
中科院分区：
文献类型：
--
作者：
Urase, Taro;Okazaki, Mitsuhiro;Tsutsui, Hirofumi
产超广谱 β-内酰胺酶 (ESBL) 的大肠杆菌和耐碳青霉烯类肠杆菌 (CRE) 的患病率日益增加，对全球健康构成威胁。对环境中这些耐药细菌的监测可以提供反映健康和感染人群的区域患病率，尽管这些耐药细菌（尤其是 CRE）的定量监测很困难，因为它们在肠杆菌科细菌总数中所占比例较低，并且可能受到具有内在耐药性的本地物种的干扰。在这项研究中，对 12 个不同处理厂处理过的废水中的这些耐药细菌进行了定量。东京氯化废水中耐头孢噻肟和产 ESBL 大肠杆菌占大肠杆菌总数的比例分别为 5.7% 和 5.3%。与克雷伯氏菌属的组成种相比，CRE 的估计比例为 0.007%。和肠杆菌属，尽管第一次培养期间的条件可能会影响估计，即使在通过分离株中耐药菌群的比例进行校正后也是如此。本研究中观察到的耐药比例不仅低于住院患者的院内感染监测，也低于门诊患者的院内感染监测，高于兽医监测。
The increasing prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and carbapenem-resistant Enterobacteriaceae (CRE) is a worldwide health threat. Monitoring of these resistant bacteria in the environment can provide regional prevalence reflecting both healthy and infected populations, although the quantitative monitoring of those resistant bacteria, especially CRE, is difficult due to their low proportion in the total Enterobacteriaceae population and the possible interference by autochthonous species with intrinsic resistance. In this study, these resistant bacteria in treated wastewater were quantified at 12 different treatment plants. The proportions of cefotaxime-resistant and ESBL-producing E. coli in the total E. coli population in the chlorinated effluents in Tokyo were 5.7 and 5.3%, respectively. The estimated proportion of CRE was 0.007% with the constituting species of Klebsiella spp. and Enterobacter spp., although the conditions during the first incubation may have affected the estimation even after the correction by the proportion of resistant population in the isolates. The observed resistant proportions in this study were lower than those in the surveillance on nosocomial infection not only for inpatients but also for outpatients, and higher than those in the veterinary monitoring.