Histone acetylation increases chromatin accessibility
Histone acetylation increases chromatin accessibility
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DOI：
10.1242/jcs.02689
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发表时间：
2005-12-15
影响因子：
4
通讯作者：
Rippe, K
中科院分区：
文献类型：
--
作者：
Görisch, SM;Wachsmuth, M;Rippe, K
在真核生物中，DNA与蛋白质和参与基因表达的超分子复合体的相互作用是由染色质的动态组织控制的，因为它决定了DNA的可及性。在这里，使用42 kDa到2.5 mda分子量的显微注射荧光素标记的葡聚糖的核分布来表征染色质的可及性依赖于组蛋白乙酰化。荧光素-葡聚糖大小的测量与图像相关光谱分析相结合，确定了三种不同的间期染色质凝聚状态，其表观孔径分别为16-20 nm、36-56 nm和60-100 nm。随着组蛋白乙酰化程度的增加，染色质的构象发生了可逆的变化，形成了均匀的60-100 nm孔径分布。这一结果确定了组蛋白乙酰化是间期染色质可及性动态调节的核心因素。在有丝分裂染色体中，染色质排斥极限为10-20 nm，与组蛋白乙酰化状态无关。
In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.