Going beyond five bases in DNA sequencing
Going beyond five bases in DNA sequencing
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DOI：
10.1016/j.sbi.2012.04.002
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发表时间：
2012-06-01
影响因子：
6.8
通讯作者：
Turner, Stephen W.
中科院分区：
文献类型：
--
作者：
Korlach, Jonas;Turner, Stephen W.
DNA测序提供了关于生物系统的丰富信息，但到目前为止，主要集中在四个典型碱基，以及通过将基因组DNA序列与亚硫酸氢盐处理后转化的四个碱基序列进行比较来获得5-甲基胞嘧啶。然而，已知许多其他对核苷酸的化学修饰可以控制基本的生命功能，影响病原体的毒力，并与许多疾病有关。这些修改不能用传统的测序方法获得。在这个观点中，我们重点介绍了几种新兴的单分子测序技术，这些技术作为测序协议的组成部分，有可能直接检测多种类型的DNA修饰。
DNA sequencing has provided a wealth of information about biological systems, but thus far has focused on the four canonical bases, and 5-methylcytosine through comparison of the genomic DNA sequence to a transformed four-base sequence obtained after treatment with bisulfite. However, numerous other chemical modifications to the nucleotides are known to control fundamental life functions, influence virulence of pathogens, and are associated with many diseases. These modifications cannot be accessed with traditional sequencing methods. In this opinion, we highlight several emerging single-molecule sequencing techniques that have the potential to directly detect many types of DNA modifications as an integral part of the sequencing protocol.