Induction of interleukin-6 and interleukin-8 gene expression by root canal sealers in human osteoblastic cells.
Induction of interleukin-6 and interleukin-8 gene expression by root canal sealers in human osteoblastic cells.
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根管封闭剂在人成骨细胞中诱导白细胞介素 6 和白细胞介素 8 基因表达。
DOI：
10.1097/01.don.0000155227.86046.a2
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发表时间：
2005
影响因子：
4.2
通讯作者：
Yu
中科院分区：
文献类型：
--
作者：
F. Huang;C. tSai;Shun;Yu
组织学研究表明，根管封闭剂可以诱发轻度至重度炎症交替。然而，关于根管封闭剂引起炎症反应的确切机制的信息很少。局部疾病部位细胞因子产生失调被认为是炎症性疾病发展的主要原因。据报道，白细胞介素 (IL)-6 和 IL-8 的释放在炎症的发病机制中发挥重要作用。本研究的目的是探讨根管封闭剂 N2（氧化锌丁香酚基）和 AH Plus（环氧树脂基）对人成骨细胞系 U2OS 细胞中 IL-6 和 IL-8 mRNA 基因表达的影响。通过半定量逆转录酶聚合酶链式反应分析测量mRNA的水平。将静止的 U2OS 细胞暴露于 N2 和 AH Plus 会诱导 IL-6 和 IL-8 mRNA 基因表达 (p < 0.05)。发现IL-8 mRNA 基因的强度显着高于IL-6 mRNA 基因(p < 0.05)。综上所述，IL-6和IL-8 mRNA基因表达的激活可能是基于氧化锌-丁子香酚和基于环氧树脂的根管封闭剂引起的根尖周炎症的发病机制之一。
Histological investigations have demonstrated that root canal sealers can induce mild to severe inflammatory alternations. However, there is little information on the precise mechanisms about root canal sealers-induced inflammatory reaction. Dysregulated cytokine productions at local disease sites have been considered to be major contributors to the development of inflammatory diseases. Interleukin (IL)-6 and IL-8 released have been reported to play an important role in the pathogenesis of inflammation. The aim of this study was to investigate the effects of root canal sealers N2 (zinc-oxide eugenol based) and AH Plus (epoxy resin based) on the expression of IL-6 and IL-8 mRNA gene in human osteoblastic cell line U2OS cells. The levels of mRNAs were measured by the semi-quantitative reverse-transcriptase polymerase chain reaction analysis. The exposure of quiescent U2OS cells to N2 and AH Plus resulted in the induction of IL-6 and IL-8 mRNA gene expression (p < 0.05). The intensity of IL-8 mRNA gene was found to be significant higher than IL-6 mRNA gene (p < 0.05). Taken together, the activation of IL-6 and IL-8 mRNA gene expression may be one of the pathogenesis of zinc oxide-eugenol based and epoxy resin based root canal sealers-induced periapical inflammation.
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Katz,Y;Strunk,RC
通讯作者：
Strunk,RC