A SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development
A SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development
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SUMO 连接酶 AtMMS21 调节根发育中 26S 蛋白酶体的活性
DOI：
10.1016/j.plantsci.2018.12.014
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Yang Chengwei
中科院分区：
文献类型：
--
作者：
Yu Mengyuan;Meng Bolun;Wang Feige;He Zhipeng;Hu Risheng;Du Jinju;Lai Jianbin;Yang Chengwei
26 S蛋白酶体是一种多亚基蛋白酶，控制大部分胞质和核蛋白的周转，调节真核生物中的许多细胞事件。然而，该复合物的功能修饰仍不清楚。在这里，我们发现了一个新的机制，SUMO连接酶AtMMS 21调节活性的26 S蛋白酶体在拟南芥根的发展。我们的体外和体内数据支持AtMMS 21与26 S蛋白酶体的亚基RPT 2a相互作用。AtMMS 21和RPT 2a的突变体表现出类似的根发育缺陷，表明它们在这一过程中存在关联。此外，RPT 2a被SUMO 3修饰，可能与AtMMS 21相关。在发育过程中，AtMMS 21和RPT 2a突变体的26 S蛋白酶体活性均低于野生型。此外，在缺乏AtMMS 21的情况下，RPT 2a的蛋白质水平而不是RNA水平降低，这意味着通过AtMMS 21-RPT 2a相互作用来调节蛋白酶体复合物的稳定性。本研究为深入了解26 S蛋白酶体在根发育过程中的调控机制提供了理论依据。
The 26S proteasome is a multi-subunit protease controlling most of the cytosolic and nuclear protein turnover, regulating many cellular events in eukaryotes. However, functional modification on this complex remains unclear. Here, we showed a novel mechanism that a SUMO ligase AtMMS21 regulates activity of the 26S proteasome in root development of Arabidopsis. Our in vitro and in vivo data supported that AtMMS21 interacts with RPT2a, a subunit of the 26S proteasome. The mutants ofAtMMS21andRPT2adisplay similar developmental defect of roots, suggesting their association in this process. In addition, RPT2a is modified by SUMO3, potentially related to AtMMS21. During development, the activity of the 26S proteasome is lower in both mutants ofAtMMS21andRPT2a, compared with that of wild type. Furthermore, the protein level but not the RNA level ofRPT2ais decreased in the absence ofAtMMS21, implying stability regulation of the proteasome complex through the AtMMS21-RPT2a interaction. Taken together, the current study would improve our understanding on the regulatory mechanism of the 26S proteasome via protein modification in root development.