Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis.
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DOI：
10.1016/j.carbpol.2015.12.050
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发表时间：
2016-04
影响因子：
11.2
通讯作者：
Junmin Wang;Beilei Ge;Zihong Li;F. Guan;Feifei Li
中科院分区：
文献类型：
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作者：
Junmin Wang;Beilei Ge;Zihong Li;F. Guan;Feifei Li
从当归中分离得到4个多糖级分（CAPS 30、CAPS 50、CAPS 70和CAPS 80）和总多糖（CAPSt）。采用傅里叶变换红外光谱（FT-IR）、高效凝胶渗透色谱（HPGPC）和气相色谱-质谱（GC-MS）对产物进行结构鉴定、分子量测定和组成分析。进一步比较了它们的体外免疫调节活性。结果表明，它们具有不同的结构特征和免疫增强活性。它们均能引起淋巴细胞增殖，上调刺激外周血淋巴细胞分泌IFN-γ、IL-2、IL-6和TNF-α。进一步的实验表明CAPS 50和CAPS 70在一定程度上提高了CD 3 + CD 56+细胞的比例。结果表明，5种CAPSs具有不同的免疫增强活性，这与它们的结构特征有关，其中分子量为20.82kDa、甘露糖和葡萄糖摩尔比为1.20：1.01的CAPS 70具有最强的免疫增强活性。
Four fractional polysaccharides (CAPS30, CAPS50, CAPS70and CAPS80) and total polysaccharides (CAPSt) were obtained fromAngelica sinensis. Their structures were identified by Fourier transform-infrared spectroscopy (FT-IR), molecular weights were evaluated by high performance gel permeation chromatography (HPGPC) and compositions were analyzed by gas chromatography–mass spectrometry (GC–MS). Their immunoregulation activities were further compared in vitro. The results showed that they displayed different structural features and immuno-enhancement activities. They all could cause the proliferation of the lymphocyte, up-regulate stimulate the productions of IFN-γ, IL-2, IL-6 and TNF-α secretion in the peripheral lymphocytes. Further experiments showed that CAPS50and CAPS70could increase the ratio of CD3+CD56+cells to some extent. These indicated that five CAPSs displayed different activities which were associated with their different structural characteristics and CAPS70, with the molecular weights of 20.82 kDa and consisting of mannose and glucose in the molar ratio of 1.20:1.01, possessed the strongest immuno-enhancement activity.