Direct and indirect effects of kisspeptin on liver oxidant and antioxidant systems in young male rats
Direct and indirect effects of kisspeptin on liver oxidant and antioxidant systems in young male rats
复制标题
DOI：
10.1002/cbf.1656
复制
发表时间：
2010-06-01
影响因子：
3.6
通讯作者：
Yilmaz, B.
中科院分区：
文献类型：
--
作者：
Aydin, M.;Oktar, S.;Yilmaz, B.
Kisspeptin是近年来发现的一种下丘脑肽，在生殖功能的中枢控制中起重要作用。本文研究了Kisspeptin对幼年雄性大鼠肝脏氧化应激的直接和间接影响。Kisspeptin-10（20 nmol/只/日）连续给药7 d。第3组大鼠仅给予一剂GnRH激动剂戈舍林（0 ~ 9 mg/只）。检测肝组织过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、黄嘌呤氧化酶（XO）、腺苷脱氨酶（AD）活性及丙二醛（MDA）含量。血清测定总抗氧化能力（TAC）、总氧化状态（TOS）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、碱性磷酸酶（ALP）、血尿素氮（BUN）、胆固醇、脂蛋白（HDL）和甘油三酯（TG）。Kisspeptin能提高SOD和CAT活性（P <005）。丙二醛的水平。TOS和AST较低，但BUN、胆固醇、HDL和AD水平在其他三组中较高（p < 0.05）。总之，我们的研究结果表明，kisspeptin可能具有抗氧化作用，从而对肝组织具有保护作用。
Kisspeptin is a recently discovered hypothalamic peptide which plays an important role in the central control of reproductive functions We have investigated direct and indirect effects of kisspeptin on the liver oxidative stress in young male rats Twenty-four rats were divided into four groups (n = 6/group) First group served as control and received saline. Kisspeptin-10 was administered to the animals in the second group (20 nmol/rat/day), for a period of 7 days Rats were given only one dose gosereline (0 9 mg/rat), a GnRH agonist in the third group The last group received kisspeptin-10 with gosereline. The activities of catalase, superoxide dismutase (SOD), xanthine oxidase (XO), adenosine deaminase (AD) and level of malondialdehyde were studied in liver tissue Serum samples were separated for total antioxidant capacity (TAC), total oxidant status (TOS), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, blood urea nitrogen (BUN), colesterol, lipoprotein (HDL) and triglyceride. Kisspeptin increased the activities of SOD and catalase (p < 0 05) When compared to the control group. the levels of malondialdehyde. TOS and AST were lower, but levels of BUN, cholesterole, HDL and AD were higher in the other three groups (p < 0.05) In conclusion, our findings suggest that kisspeptin may have antioxidant and thus protective effects on the liver tissue Copyright (C) 2010 John Wiley & Sons, Ltd