A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology.
A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology.
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DOI：
10.1038/srep27818
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发表时间：
2016-06-10
影响因子：
4.6
通讯作者：
Suh M
中科院分区：
文献类型：
--
作者：
Heo C;Park H;Kim YT;Baeg E;Kim YH;Kim SG;Suh M
慢性体内成像和电生理学对于更好地理解神经功能和电路是重要的。我们介绍了新的颅窗使用软，可穿透，弹性，透明，硅基聚二甲基硅氧烷（PDMS）作为替代品的头骨和硬脑膜在大鼠和小鼠。PDMS可以很容易地定制成任何尺寸和形状，以覆盖大的大脑区域。植入后15周观察到清晰和健康的皮质血管。在感觉刺激期间成功地监测了实时血流动力学反应。此外，PDMS窗口允许将微电极和微量移液器容易地插入皮质组织中，用于在任何位置进行电生理记录和化学注射，而不会引起任何液体泄漏。Cx 3Cr 1 +/− GFP转基因小鼠的纵向双光子显微镜成像与通过传统玻璃型颅窗的成像相当，即使在直接皮质内注射后立即进行。这个颅骨窗口将有助于长期大脑研究的直接探测和映射。
Chronic in vivo imaging and electrophysiology are important for better understanding of neural functions and circuits. We introduce the new cranial window using soft, penetrable, elastic, and transparent, silicone-based polydimethylsiloxane (PDMS) as a substitute for the skull and dura in both rats and mice. The PDMS can be readily tailored to any size and shape to cover large brain area. Clear and healthy cortical vasculatures were observed up to 15 weeks post-implantation. Real-time hemodynamic responses were successfully monitored during sensory stimulation. Furthermore, the PDMS window allowed for easy insertion of microelectrodes and micropipettes into the cortical tissue for electrophysiological recording and chemical injection at any location without causing any fluid leakage. Longitudinal two-photon microscopic imaging of Cx3Cr1+/− GFP transgenic mice was comparable with imaging via a conventional glass-type cranial window, even immediately following direct intracortical injection. This cranial window will facilitate direct probing and mapping for long-term brain studies.