Detached shoot treatments cannot replace whole-tree assays when phenotyping for apple resistance to Neonectria ditissima
Detached shoot treatments cannot replace whole-tree assays when phenotyping for apple resistance to Neonectria ditissima
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在对苹果对新克霉的抗性进行表型分析时，离体枝条处理不能替代整树测定
DOI：
10.30843/nzpp.2018.71.137
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发表时间：
2018
影响因子：
--
通讯作者：
D. Hedderley
中科院分区：
文献类型：
--
作者：
R. Scheper;B. M. Fisher;T. Taylor;D. Hedderley
为了建立一种快速的苹果欧洲溃疡病抗性表型分析方法，将分离茎法与全树表型分析方法进行了比较。在温室中比较了6份苹果材料的离体苗和盆栽树接种新树苗后的病害发病率和病害长度。分离的芽被放置在曝气的水中或在蝶蛹溶液中，有和没有水的变化。“Royal Gala”树的发病率最高（90%），而“Robusta 5”树仍然没有病变。处理-加入互作效应显著，‘罗布斯塔5号’离体梢的发病率（4-32%）高于‘Braeburn’（0-2%），而‘Braeburn’树的发病率较高（64%）。“罗布斯塔5号”的病变比其他品种小，但在三个评估日期中，只有一个品种之间存在显著差异。在使用疾病发病率或病变长度时，与盆栽树试验相比，没有一种离枝试验得出类似的排名。在对苹果的欧洲溃疡病抗性进行表型分析时，离枝处理不能代替整株试验。
To develop a fast phenotyping method for European canker resistance of apple, detached-shoot assays were compared with whole-tree phenotyping methods. Disease incidence and lesion length in detached shoots and potted trees of six apple accessions were compared after inoculation with Neonectria ditissima conidia in a glasshouse. Detached shoots were placed in aerated water or in a Chrysal solution, with and without water changes. ‘Royal Gala’ trees had the highest disease incidence (90%) and ‘Robusta 5’ trees remained lesion-free. The treatment-accession interaction effect was significant, with higher disease incidence in detached shoots of ‘Robusta 5’ (4—32%) than ‘Braeburn’ (0—2%), while ‘Braeburn’ trees had a high disease incidence (64%). ‘Robusta 5’ had smaller lesions than the other accessions, but significant differences between accessions were observed on only one of three assessment dates. None of the detached-shoot assays resulted in a similar ranking of the accessions compared with the potted-tree assay, using disease incidence or lesion length. Detached-shoot treatments cannot replace whole-tree assays when phenotyping for European canker resistance of apple.