SARS-CoV spike protein-expressing recombinant vaccinia virus efficiently induces neutralizing antibodies in rabbits pre-immunized with vaccinia virus
SARS-CoV spike protein-expressing recombinant vaccinia virus efficiently induces neutralizing antibodies in rabbits pre-immunized with vaccinia virus
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DOI：
10.1016/j.vaccine.2006.08.039
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发表时间：
2007-01-08
期刊：
影响因子：
5.5
通讯作者：
Kohara, Michinori
中科院分区：
文献类型：
--
作者：
Kitabatake, Masahiro;Inoue, Shingo;Kohara, Michinori
一种针对严重急性呼吸综合征(SARS)的疫苗正在紧锣密鼓地研究，以防止其再次出现。我们用高效减毒株LC16m8构建了表达SARS冠状病毒刺突蛋白的重组痘苗病毒(RVV-S)。兔皮内注射轮状病毒S 1周后，可诱导产生抗SARS-CoV的中和抗体，加强免疫后，NT效价可达1：1000。值得注意的是，RVV-S可诱导预先免疫的兔产生抗SARS-CoV的NT抗体。轮状病毒S在存在抗冠状病毒NT抗体的情况下，仍能诱导产生抗SARS冠状病毒的NT抗体。这些结果表明，轮状病毒S可能是一种强大的SARS疫苗，包括在以前接种过天花疫苗的患者中。(C)2006爱思唯尔有限公司。保留所有权利。
A vaccine for severe acute respiratory syndrome (SARS) is being intensively pursued against its re-emergence. We generated a SARS coronavirus (SARS-CoV) spike protein-expressing recombinant vaccinia virus (RVV-S) using highly attenuated strain LC16m8. Intradermal administration of RVV-S into rabbits induced neutralizing (NT) antibodies against SARS-CoV I week after administration and the NT titer reached 1: 1000 after boost immunization with RVV-S. Significantly, NT antibodies against SARS-CoV were induced by administration of RVV-S to rabbits that had been pre-immunized with LC 16m8. RVV-S can induce NT antibodies against SARS-CoV despite the presence of NT antibodies against VV. These results suggest that RVV-S may be a powerful SARS vaccine, including in patients previously immunized with the smallpox vaccine. (c) 2006 Elsevier Ltd. All rights reserved.