Effects of intranasal inoculation with Bordetella bronchiseptica, porcine reproductive and respiratory syndrome virus, or a combination of both organisms on subsequent infection with Pasteurella multocida in pigs
Effects of intranasal inoculation with Bordetella bronchiseptica, porcine reproductive and respiratory syndrome virus, or a combination of both organisms on subsequent infection with Pasteurella multocida in pigs
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DOI：
10.2460/ajvr.2001.62.521
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发表时间：
2001-04-01
影响因子：
1
通讯作者：
Rimler, RB
中科院分区：
文献类型：
--
作者：
Brockmeier, SL;Palmer, MV;Rimler, RB
试验1-确定鼻内接种猪繁殖与呼吸综合征病毒（PRRSV）或支气管败血性博德特氏菌对猪中非致病性多杀性巴氏杆菌（Pasteurella multocida）攻击的影响。动物-70头3周龄猪。程序-在试验1中，猪不接种[n = 10]或接种PRRSV（10）、多杀性巴氏杆菌（10）或PRRSV随后用多杀性巴氏杆菌（10）攻击。在实验2中，猪未接种（n = 10）或接种B支气管败血症（10）或PRRSV和B支气管败血症（10）;所有猪均用多杀性巴斯德菌攻毒。每组5头猪进行尸检14和21天后，初始inoculations.Results-Pasteurella multocida没有分离与P multocida单独或接种后与PRRSV挑战的猪的组织标本。然而，在接种支气管败血症B菌后进行攻毒的猪中，从鼻腔和软腭扁桃体标本中分离出多杀性巴斯德菌。B bronchiseptica。在猪中分离出的细菌数量增加后，共同接种PRRSV和B bronchiseptica的挑战，所有3个代理商被隔离，从这些pigs.Conclusions和临床相关性感染的猪与B bronchiseptica，但不是PRRSV之前的挑战与P multocida导致定植的上呼吸道和扁桃体的软腭与P multocida。PRRSV和B支气管败血症的共感染使猪易于感染上呼吸道和肺部的多杀性巴斯德菌。猪繁殖与呼吸综合征病毒和B支气管败血症可能相互作用，对呼吸道防御机制产生不利影响，使猪特别容易感染继发性病原体，如多杀性巴斯德氏菌。
Objective-To determine effects of intranasal inoculation with porcine reproductive and respiratory syndrome virus (PRRSV) or Bordetella bronchiseptica on challenge with nontoxigenic Pasteurella multocida in pigs.Animals-Seventy 3-week-old pigs.Procedure-In experiment 1, pigs were not inoculated [n = 10) or were inoculated with PRRSV (10), P multocida (10), or PRRSV followed by challenge with P multocida (10). In experiment 2, pigs were not inoculated (n = 10) or were inoculated with B bronchiseptica (10) or PRRSV and B bronchiseptica (10); all pigs were challenged with P multocida. Five pigs from each group were necropsied 14 and 21 days after initial inoculations.Results-Pasteurella multocida was not isolated from tissue specimens of pigs challenged with P multocida alone or after inoculation with PRRSV. However, in pigs challenged after inoculation with B bronchiseptica, P multocida was isolated from specimens of the nasal cavity and tonsil of the soft palate. Number of bacteria isolated increased in pigs challenged after coinoculation with PRRSV and B bronchiseptica, and all 3 agents were isolated from pneumonic lesions in these pigs.Conclusions and Clinical Relevance-Infection of pigs with B bronchiseptica but not PRRSV prior to challenge with P multocida resulted in colonization of the upper respiratory tract and tonsil of the soft palate with P multocida. Coinfection with PRRSV and B bronchiseptica predisposed pigs to infection of the upper respiratory tract and lung with P multocida. Porcine reproductive and respiratory syndrome virus and B bronchiseptica may interact to adversely affect respiratory tract defense mechanisms, leaving pigs especially vulnerable to infection with secondary agents such as P multocida.