Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model
Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model
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DOI：
10.3233/bme-201172
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发表时间：
2021-01-01
影响因子：
1
通讯作者：
Takaso, Masashi
中科院分区：
文献类型：
--
作者：
Shoji, Shintaro;Uchida, Kentaro;Takaso, Masashi
背景：利用过氧化氢和辣根过氧化物酶的酶交联策略在原位形成的水凝胶（IFHGs）中的应用正受到越来越多的关注。ifhg也可能是骨修复的理想载体材料，尽管它们携带骨形态发生蛋白-2 （BMP2）的能力还有待研究。目的：我们研究了含有BMP2的透明质酸（IFHG- ha）制成的IFHG在小鼠临界大小骨缺损模型中促进骨形成的有效性。方法：C57/BL6J小鼠接受2 mm股骨临界骨缺损后，随机分为以下治疗组（n = 6）：对照组（不治疗）、IFHG-HA组、PBS + BMP2组和IFHG-HA + BMP2组。利用x线片追踪新生骨形成，术后2周对骨缺损部位新生骨进行显微计算机断层扫描和组织学检查。结果：PBS加BMP2和IFHG-HA加BMP2处理小鼠骨体积（BV）和骨矿物质含量（BMC）均高于对照组，实现了骨实变。IFHG-HA加BMP2处理小鼠的BV和BMC明显高于PBS加BMP2处理小鼠。结论：IFHG-HA可能是BMP2的有效载体，可用于骨缺损修复。
BACKGROUND: An enzymatic crosslinking strategy using hydrogen peroxide and horseradish peroxidase is receiving increasing attention for application with in situ-formed hydrogels (IFHGs). IFHGs may also be ideal carrier materials for bone repair, although their ability to carry bone morphogenetic protein-2 (BMP2) has yet to be examined.OBJECTIVE: We examined the effectiveness of an IFHG made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model.METHODS: C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n = 6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery.RESULTS: Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2.CONCLUSIONS: IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.