Salivary immunoglobulin A directed to oral microbial GroEL in patients with periodontitis and their potential protective role
Salivary immunoglobulin A directed to oral microbial GroEL in patients with periodontitis and their potential protective role
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DOI：
10.1111/j.1399-302x.2006.00290.x
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发表时间：
2006-10-01
影响因子：
--
通讯作者：
Yoshioka, M.
中科院分区：
文献类型：
--
作者：
Fukui, M.;Hinode, D.;Yoshioka, M.
本研究的目的是确定针对牙周炎患者口腔微生物GroEL的唾液免疫球蛋白A(IgA)，并通过减少微生物GroEL诱导的炎症细胞因子的产生来证明其潜在的保护作用。使用属于热休克蛋白60家族的五种不同蛋白，对63名受试者唾液中的IgA进行免疫印迹分析，发现与对照组相比，牙周炎患者唾液中的直弯杆菌GroEL和牙龈卟啉单胞菌GroEL具有免疫反应性(P<；0.05)。用Biacore 1000测定唾液中针对直肠弯曲菌的IgA滴度，发现牙周炎患者唾液中有较高的共振单位(RU)值(P<；0.01)。此外，深窝深度(>；=5 mm)的牙数与RU值呈高度相关(r=0.50，P<；0.01)。直肌具有刺激牙龈成纤维细胞产生白介素6的作用。有趣的是，针对直肠弓形虫的唾液IgA抗体可部分抑制白介素6的产生。这项研究表明，与GroEL有关的高水平唾液IgA与牙周病严重程度之间存在关系。虽然还需要进一步的研究，但唾液中的IgA对GroEL可能具有保护作用，它可以减少牙周致病细菌来源的GroEL诱导的炎症反应。
The aim of this study was to identify salivary immunoglobulin A (IgA) directed to oral microbial GroEL in patients with periodontitis and to demonstrate their potential protective role through a reduction of inflammatory cytokine production induced by microbial GroEL. Using five different proteins belonging to the heat-shock protein 60 family, Western immunoblot analysis of salivary IgA from 63 subjects revealed immunoreactivities with Campylobacter rectus GroEL and Porphyromonas gingivalis GroEL in subjects with periodontitis (P < 0.05) compared to control subjects. Using the BIACORE 1000 to measure the salivary IgA titers directed towards C. rectus GroEL, high resonance unit (RU) values were observed in the saliva samples from patients with periodontitis (P < 0.01). Furthermore, the number of teeth with deep pocket depth (>= 5 mm) showed a high correlation coefficient with the RU value (r = 0.50, P < 0.01). C. rectus GroEL possessed the ability to stimulate the production of interleukin-6 by gingival fibroblasts. Interestingly, salivary IgA antibody directed to C. rectus GroEL caused a partial inhibition of interleukin-6 production. This study showed a relationship between high levels of salivary IgA directed to GroEL and periodontal disease severity. Although additional investigations are required, salivary IgA to GroEL may have a protective role by reducing the inflammatory response induced by GroEL derived from periodontopathogenic bacteria.