A framework for identifying fertility gene targets for mammalian pest control.
A framework for identifying fertility gene targets for mammalian pest control.
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确定哺乳动物害虫控制的生育基因目标的框架。
DOI：
10.1101/2023.05.30.542751
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Gemmell,NeilJ
中科院分区：
文献类型：
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作者：
Clark,AnnaC;Alexander,Alana;Edison,Rey;Esvelt,Kevin;Kamau,Sebastian;Dutoit,Ludovic;Champer,Jackson;Champer,SamuelE;Messer,PhilippW;Gemmell,NeilJ
以生育为目标的基因驱动已被提议作为一种伦理遗传方法，用于管理野生脊椎动物害虫种群，以利于公共健康和保护。这篇手稿介绍了一种基于生物基因功能、基因表达和小鼠基因敲除模型的结果来识别和评估目标基因适合性的框架。这一框架确定了16个对男性生育能力至关重要的基因和12个对女性生育能力重要的基因，这些基因可能是哺乳动物基因驱动和其他非驱动基因害虫控制技术的可行靶标。此外，比较基因组学分析表明，在几种具有全球意义的入侵哺乳动物中，已确定的基因是保守的。除了为确定候选基因提供重要的考虑因素外，我们的框架和本研究中确定的基因可能在开发其他害虫控制工具(如野生动物避孕药)方面有实用价值。
Fertility‐targeted gene drives have been proposed as an ethical genetic approach for managing wild populations of vertebrate pests for public health and conservation benefit. This manuscript introduces a framework to identify and evaluate target gene suitability based on biological gene function, gene expression and results from mouse knockout models. This framework identified 16 genes essential for male fertility and 12 genes important for female fertility that may be feasible targets for mammalian gene drives and other non‐drive genetic pest control technology. Further, a comparative genomics analysis demonstrates the conservation of the identified genes across several globally significant invasive mammals. In addition to providing important considerations for identifying candidate genes, our framework and the genes identified in this study may have utility in developing additional pest control tools such as wildlife contraceptives.