Requirement for IFN gamma in potentiation of interferon's antiviral and anticellular activities: identity of mouse and human systems.
Requirement for IFN gamma in potentiation of interferon's antiviral and anticellular activities: identity of mouse and human systems.
复制标题
增强干扰素抗病毒和抗细胞活性所需的干扰素γ：小鼠和人类系统的特性。
DOI：
10.1089/jir.1984.4.265
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Fleischmann,CM
中科院分区：
文献类型：
--
作者：
FleischmannJr,WR;Schwarz,LA;Fleischmann,CM
增强作用最初被证明是在含有MuIFNα/β和MuIFN-γ的混合制剂的小鼠系统中干扰素（IFN）活性的非累加性协同增强。现在已经研究了小鼠和人类系统的抗病毒和抗细胞活性的增强，并且在两种系统中IFN-α和IFN-β与IFN-γ协同相互作用，但彼此不相互作用。IFN-γ对IFN-α和IFN-β的抗病毒和抗细胞活性有同等的增强作用。HuIFN的比活性为107 U/mg蛋白质或更高，证明了其增强作用。天然产生的和重组HuIFN-αs具有相同的被HuIFN-γ增强的相对能力。结论是，IFN-γ与IFN-α或IFN-β一起对于增强是必需的，抗细胞和抗病毒作用的增强以类似的方式发生，并且小鼠和人类系统中的增强之间存在密切的相关性。这些结果表明，增强是由两种不同的IFN类型（免疫与病毒型）的相互作用引起的，小鼠中的增强研究可能与人类直接相关。
Potentiation was originally demonstrated as a nonadditive, synergistic enhancement of interferon (IFN) activity in the mouse system for mixed preparations containing MuIFNα/β and MuIFN-γ. Potentiation of the antiviral and anticellular activities has now been studied for mouse and human systems, and in both systems IFN-α and IFN-β interacted synergistically with IFN-γ, but not with each other. Further, the antiviral and anticellular activities of IFN-α and IFN-β were potentiated equally by IFN-γ. Potentiation was demonstrated for HuIFNs with specific activities of 107U/mg of protein and higher. Naturally produced and recombinant HuIFN-αs had the same relative abilities to be potentiated by HuIFN-γ. It was concluded that IFN-γ with either IFN-α or IFN-β was essential for potentiation, that potentiation of anticellular and antiviral actions occurred in similar manners, and that a close correlation existed between potentiation in mouse and human systems. These results suggest that potentiation was caused by the interaction of two dissimilar IFN types (immune versus virus-type) and that potentiation studies in the mouse may be directly relevant for humans.