Intestinal cellular heterogeneity and disease development revealed by single-cell technology.
Intestinal cellular heterogeneity and disease development revealed by single-cell technology.
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单细胞技术揭示肠道细胞异质性和疾病发展
DOI：
10.1186/s13619-022-00127-6
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发表时间：
2022-09-01
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肠上皮负责食物消化和营养吸收，并在激素分泌、微生物防御和免疫反应中起关键作用。这些功能依赖于完整的单层肠上皮，其具有多样的细胞组成和快速的自我更新，并在损伤后表现出强大的再生可塑性。肠上皮细胞的稳态失调导致疾病的发展，最常见的包括肠炎和结肠直肠癌。因此，在分子水平上了解肠上皮细胞的特性及其维持稳态的机制是非常重要的。单细胞技术使我们能够在单细胞水平上获得分子见解。本文综述了单细胞RNA测序在了解肠道细胞特征、时空演变和肠道疾病发展中的应用。
The intestinal epithelium is responsible for food digestion and nutrient absorption and plays a critical role in hormone secretion, microorganism defense, and immune response. These functions depend on the integral single-layered intestinal epithelium, which shows diversified cell constitution and rapid self-renewal and presents powerful regeneration plasticity after injury. Derailment of homeostasis of the intestine epithelium leads to the development of diseases, most commonly including enteritis and colorectal cancer. Therefore, it is important to understand the cellular characterization of the intestinal epithelium at the molecular level and the mechanisms underlying its homeostatic maintenance. Single-cell technologies allow us to gain molecular insights at the single-cell level. In this review, we summarize the single-cell RNA sequencing applications to understand intestinal cell characteristics, spatiotemporal evolution, and intestinal disease development.
DOI：
10.1186/s13619-021-00085-5
发表时间：
2021-07-06
期刊：
Cell regeneration (London, England)
影响因子：
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作者：
Hutchins AP
通讯作者：
Hutchins AP