Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles
Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles
复制标题
DOI：
10.1261/rna.028639.111
复制
发表时间：
2012-04-01
期刊：
影响因子：
4.5
通讯作者：
Akimitsu, Nobuyoshi
中科院分区：
文献类型：
--
作者：
Miyagawa, Ryu;Tano, Keiko;Akimitsu, Nobuyoshi
MALAT-1非编码RNA定位于核斑点，尽管其mRNA样特征。在这里，我们报告的几个关键因素，促进本地化的MALAT-1的核斑点的识别，也提供了证据表明，MALAT-1参与基因表达的调控。异核体分析表明，MALAT-1不穿梭于细胞核和细胞质之间。RNAi介导的核斑点蛋白RNPS 1、SRm 160或IBP 160（它们是众所周知的mRNA加工因子）的抑制导致MALAT-1扩散到核质。我们证明，MALAT-1包含两个不同的元素，指导转录核斑点，这也能够结合RNPS 1在体外。MALAT-1的缺失抑制了几个基因的表达。总之，我们的研究结果表明RNPS 1，SRm 160和IBP 160有助于MALAT-1定位于核斑点，其中MALAT-1可能参与调节基因表达。
MALAT-1 noncoding RNA is localized to nuclear speckles despite its mRNA-like characteristics. Here, we report the identification of several key factors that promote the localization of MALAT-1 to nuclear speckles and also provide evidence that MALAT-1 is involved in the regulation of gene expression. Heterokaryon assays revealed that MALAT-1 does not shuttle between the nucleus and cytoplasm. RNAi-mediated repression of the nuclear speckle proteins, RNPS1, SRm160, or IBP160, which are well-known mRNA processing factors, resulted in the diffusion of MALAT-1 to the nucleoplasm. We demonstrated that MALAT-1 contains two distinct elements directing transcripts to nuclear speckles, which were also capable of binding to RNPS1 in vitro. Depletion of MALAT-1 represses the expression of several genes. Taken together, our results suggest that RNPS1, SRm160, and IBP160 contribute to the localization of MALAT-1 to nuclear speckles, where MALAT-1 could be involved in regulating gene expression.