HYDRODYNAMIC ANALYSIS OF EGG PHOSPHATIDYLCHOLINE VESICLES
HYDRODYNAMIC ANALYSIS OF EGG PHOSPHATIDYLCHOLINE VESICLES
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鸡蛋磷脂酰胆碱囊泡的流体动力学分析
DOI：
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发表时间：
1978
影响因子：
5.2
通讯作者：
C. Huang
中科院分区：
文献类型：
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作者：
J. T. Mason;C. Huang
球形单层磷脂双层囊泡作为研究脂质双层、脂质蛋白相互作用的模型系统，以及最近可能应用于内科的药物载体，已获得广泛认可。1-6 了解单层囊泡的物理特性促进了许多此类研究，为此，流体动力学分析能够提供有关囊泡重量、形状和水合的信息，对于阐明这些物理特性非常重要。本文的目的首先综述大小均匀的鸡蛋磷脂酰胆碱囊泡的制备以及应用流体动力学表征其物理性质。然后，如下所述，将通过考虑我们实验室最近研究的囊泡行为的一个方面来说明这种对囊泡的应用。最近的研究表明，构成囊泡双层的两个单层在许多物理特性方面是不对称的。4，？其中一个特征似乎是构成囊泡双层的脂质分子的几何堆积。通过脂质堆积几何形状，我们不仅指这两个单层内磷脂分子可用的体积，还指平均卵磷脂酰胆碱分子在两个单层空间内的该体积的分布。通过考虑囊泡的小曲率半径8（通常
Spherical unilamellar phospholipid bilayer vesicles have gained wide acceptance as model systems for the study of lipid bilayers, lipid protein interactions, and recently as carriers of drugs with possible applications to internal medicine.1-6 Many of these studies have been facilitated by a knowledge of the physical properties of the unilamellar vesicles and to this end hydrodynamic analysis, with its ability to provide information regarding vesicle weight, shape, and hydration, has been important in the illumination of these physical characteristics. The purpose of this paper is first to review the preparation of homogeneously sized egg phosphatidylcholine vesicles and the application of hydrodynamics to characterize their physical properties. Then this application to vesicles will be illustrated, as discussed below, by considering an aspect of vesicle behavior recently under investigation in our laboratory. Recent studies have demonstrated that the two monolayers comprising the vesicle bilayer are asymmetric with respect to many of their physical properties.4, ? One such characteristic appears to be the geometric packing of the lipid molecules that make up the vesicle bilayer. By lipid packing geometry, we refer not only to the volume available to the phospholipid molecules within these two monolayers but also to the distribution of this volume within the space of the two monolayers for the averaged egg phosphatidylcholine molecules. This asymmetric packing geometry may be anticipated by a consideration of the constraint placed upon the vesicle by its small radius of curvature 8, (generally