The homogenous polysaccharide SY01-23 purified from leaf of Morus alba L. has bioactivity on human gut Bacteroides ovatus and Bacteroides cellulosilyticus
The homogenous polysaccharide SY01-23 purified from leaf of Morus alba L. has bioactivity on human gut Bacteroides ovatus and Bacteroides cellulosilyticus
复制标题
从桑树叶中纯化得到的均质多糖SY01-23对人肠道卵形拟杆菌和解纤维素拟杆菌具有生物活性
DOI：
10.1016/j.ijbiomac.2020.05.009
复制
发表时间：
2020
影响因子：
8.2
通讯作者：
Ding Kan
中科院分区：
文献类型：
--
作者：
Wang Yeqing;Shao Saicong;Guo Ciliang;Zhang Shihai;Li Meixia;Ding Kan
桑叶的药理作用多糖具有抗肿瘤、免疫刺激和抗炎等作用。然而，到目前为止，对人体肠道微生物区系的生物活性尚不清楚。从桑叶中分离得到3种均一多糖，分别为SY01-21、SY01-22和SY01-23，分子量分别为57 kDa、25 kDa和7.2 kDa。SY01-21的单糖组成为鼠李糖、半乳糖和阿拉伯糖，摩尔比为7.60：43.52：48.88。SY01-22含有鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖，摩尔比为14.61：9.06：1.35：34.65：2.99：37.34。SY01-23含有鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖，摩尔比为23.00：4.12：24.60：5.74：17.28：1.12：24.13。生物活性测定表明，SY01-21对类纤维素硅杆菌(BC)生长有促进作用，而SY01-22对卵形拟杆菌(BO)生长有促进作用。有趣的是，SY01-23对BOB和BC的生长都有促进作用。而拟杆菌(Bt)仅在5 mg/mLSY01-21上生长。耐人寻味的是，BT与BOB或BC共培养的生长情况好于单独培养。这表明它们之间可能存在交叉摄食。此外，我们还发现利用SY01-23产乙酸乙酯和丙酸。以上结果提示，SY01-23可能通过促进类杆菌在肠道内的定植而改变人的肠道微生物区系，从而改善肠道健康。
Function of mulberry leaf (Morus albaL.) polysaccharide has been reported on antitumor, immunostimulatory and anti-inflammatory effects. However, the bioactivity on human gut microbiota is unclear so far. Here, three homogenous polysaccharides named SY01-21, SY01-22, SY01-23 were isolated from mulberry leaf with molecular weight 57 kDa, 25 kDa and 7.2 kDa, respectively. The monosaccharide composition of SY01-21 contained rhamnose, galactose and arabinose in a molar ratio of 7.60:43.52:48.88. SY01-22 contained rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 14.61:9.06:1.35:34.65:2.99:37.34. SY01-23 contained rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 23.00:4.12:24.60:5.74:17.28:1.12:24.13. Bioactivity test showed SY01-21 promoted the growth ofBacteroidescellulosilyticus(BC) while SY01-22 benefited the growth ofBacteroides ovatus(BO). Interestingly, SY01-23 boosted the growth of bothBOandBC. However,Bacteroides thetaiotamicron(BT) only grew on 5 mg/mL SY01-21. Intriguingly, the growth of co-culture ofBTwithBOorBCwas better than monoculture. This suggested that cross-feeding might exist between them. Besides, we foundBOandBCgenerated acetate and propionate by utilizing SY01-23. The above results suggested that SY01-23 might modify human gut microbiota by driving colonization ofBacteroidesin the gut to improve wellness.