Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential
Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential
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DOI：
10.1242/dev.01295
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发表时间：
2004-09-01
期刊：
影响因子：
4.6
通讯作者：
Verdi, JM
中科院分区：
文献类型：
--
作者：
Bani-Yaghoub, M;Kendall, SE;Verdi, JM
神经干细胞（NSC）的非神经分化的报告已经受到了挑战，扩大分化潜力的替代解释。为了证明NSC的可塑性，从单个逆转录病毒标记的胚胎皮质前体产生神经球。在一个确定的无血清含胰岛素的培养基中，40%的神经球含有肌源性和神经源性分化的后代。显示多谱系分化潜力的神经干细胞的数量在妊娠期下降，但在成年动物中确实存在。在该系统中，胰岛素似乎作为多谱系神经干细胞（MLNSC）的存活和剂量依赖性肌源性分化信号发挥作用。MLNSCs衍生的心肌细胞同步收缩，对交感神经和副交感神经刺激作出反应，并再生受损的心脏组织。这些研究为MLNSCs在动物的整个生命周期中存在的假设提供了支持，并可能为神经系统内外基于细胞的再生医学策略提供干细胞群体。
Reports of non-neural differentiation of neural stem cells (NSCs) have been challenged by alternative explanations for expanded differentiation potentials. In an attempt to demonstrate the plasticity of NSC, neurospheres were generated from single retrovirally labeled embryonic cortical precursors. In a defined serum-free insulin-containing media, 40% of the neurospheres contained both myogenic and neurogenic differentiated progeny. The number of NSCs displaying multilineage differentiation potential declines through gestation but does exist in the adult animal. In this system, insulin appears to function as a survival and dose-dependent myogenic differentiation signal for multilineage NSCs (MLNSC). MLNSC-derived cardiomyocytes contract synchronously, respond to sympathetic and parasympathetic stimulation, and regenerate injured heart tissues. These studies provide support for the hypothesis that MLNSCs exist throughout the lifetime of the animal, and potentially provide a population of stem cells for cell-based regenerative medicine strategies inside and outside of the nervous system.